Wilson病认知功能障碍与肠道菌群的相关性研究

Association Between Cognitive Impairment and Intestinal Flora in Wilson's Disease

目的 探讨Wilson病(Wilson’s disease, WD)认知功能障碍与肠道菌群的关系,以期为临床治疗WD合并认知功能障碍提供理论基础。方法 选取38例WD伴认知功能障碍的患者作为观察组,同时选取32例WD不伴认知功能障碍的患者为对照组,取大便样品进行16S rRNA基因测序技术分析,通过检测两组患者肠道菌群的组成及其丰度,探讨两者之间的相关性。结果 在门水平上,与对照组比较,观察组拟杆菌门和放线菌门丰度明显升高(P<0.05),厚壁菌门和变形菌门丰度明显降低(P<0.05);在科水平上,与对照组比较,观察组双歧杆菌科、紫单胞菌科、拟杆菌科、产碱杆菌科、理研菌科、乳酸菌科、韦荣氏球菌科丰度明显升高(P<0.05),梭菌科、科里杆菌科、未分类拟杆菌、肠杆菌科、毛螺菌科、乳球菌科、普氏菌科、链球菌科丰度明显降低(P<0.05);在属水平上,与对照组比较,观察组乳酸菌属、小杆菌属、乳球菌属、柯林斯氏菌属、另枝菌属、拟杆菌属、双歧杆菌属、帕拉斯氏菌属丰度明显升高(P<0.05),直肠真杆菌属、布劳特氏菌属、克雷伯氏菌属、栖粪杆菌属、普雷沃氏菌属、链球菌属丰度明显降低(P<0.05)。结论 WD认知功能障碍与肠道菌群之间存在一定的相关性。

Objective To investigate the association between cognitive impairment and intestinal flora in Wilson's disease(WD),and to provide a theoretical basis for the clinical treatment of WD with cognitive impairment.Methods A total of 38 WD patients with cognitive impairment were enrolled as observation group,and 32 WD patients without cognitive impairment were enrolled as control group.Fecal samples were collected for 16S rRNA sequencing,and the association between cognitive impairment and intestinal flora was analyzed by measuring the composition and abundance of intestinal flora for both groups.Results At the phylum level,compared with the control group,the observation group had significant increases in the abundance of Bacteroidetes and Actinobacteria(P<0.05)and significant reductions in the abundance of Firmicutes and Proteobacteria(P<0.05).At the family level,compared with the control group,the observation group had significant increases in the abundance of Bifidobacteriaceae,Porphyromonadaceae,Bacteroidaceae,Alcaligenaceae,Rikenellaceae,Lactobacillaceae,and Veillonellaceae(P<0.05)and significant reductions in the abundance of Clostridiaceae,Coriobacteriaceae,Bacteroidales_S24-7_group,Enterobacteriaceae,Lachnospiraceae,Ruminococcaceae,Prevotellaceae,and Streptococcaceae(P<0.05).At the genus level,compared with the control group,the observation group had significant increases in the abundance of Lactobacillus,Dialister,Ruminococcus_2,Parabacteroides,Alistipes,Bacteroides,Bifidobacterium,and Parasutterella(P<0.05)and significant reductions in the abundance of[Eubacterium]_rectale_group,Blautia,Klebsiella,Faecalibacterium,Prevotella_9,and Streptococcus(P<0.05).Conclusion There is a certain association between cognitive impairment and intestinal flora in WD.