中线蛋白2在胃癌中表达上调并促进胃癌细胞恶性表型

Midline 2 expression upregulates in gastric cancer and promotes malignant phenotype of gastric cancer cells

目的:研究中线蛋白2(MID2)在胃癌中的表达水平及对胃癌患者预后的影响,探讨MID2表达对胃癌细胞生物学表型的影响。方法:收集2014年1月至2016年12月温州医科大学附属第一医院胃癌根治术后患者组织标本420例,联合TCGA数据库评估MID2在胃癌组织中的表达情况及对胃癌患者预后的影响。构建靶向MID2基因的过表达质粒和小干扰RNA(siRNA),转染胃癌细胞后通过CCK-8实验评估胃癌细胞的增殖能力,Transwell实验评估胃癌细胞的迁移与侵袭能力。结果:MID2表达水平在胃癌组织中上调(P<0.05);MID2高表达预示着胃癌患者较短的总体生存期(P=0.002)、无病生存期(P=0.002)、无进展生存期(P=0.003)。上调MID2的表达可以显著促进胃癌细胞BGC-823和SGC-7901的增殖和迁移、侵袭能力(P<0.05);敲低MID2能够显著抑制胃癌细胞MGC-803的增殖和迁移、侵袭能力(P<0.05)。结论:MID2在胃癌组织中表达上调,MID2高表达与胃癌患者较差的预后相关,上调MID2的表达可以显著促进胃癌细胞恶性表型。

Objective:To analyze the expression level of midline 2(MID2)in gastric cancer and its effect on the prognosis of gastric cancer patients.To investigate the effect of MID2 expression on the biological phenotype of gastric cancer cells.Methods:A total of 420 tissue samples from the First Affiliated Hospital of Wenzhou Medical University after radical gastrectomy from January 2014 to December 2016 were retrospectively collected.The expression of MID2 in gastric cancer tissues and its effect on the prognosis of gastric cancer patients were evaluated by using gastric cancer tissue samples and TCGA database.Overexpression plasmids and small interfering RNAs(siRNAs)targeting MID2 genes were constructed,and the proliferation ability of gastric cancer cells was evaluated by CCK-8 experiment after transfection of gastric cancer cells while the migration and invasion ability of gastric cancer cells was evaluated by Transwell experiment.Results:The expression level of MID2 was upregulated in gastric cancer tissues(P<0.05).High MID2 expression predicted poorer overall survival(P=0.002),disease free survival(P=0.002)and progression free survival(P=0.003)in gastric cancer patients.Upregulating the expression of MID2 significantly promoted the proliferation,migration and invasion of gastric cancer cells BGC-823 and SGC-7901(P<0.05).Knockdown MID2 could significantly inhibit the proliferation,migration and invasion of gastric cancer cells MGC-803(P<0.05).Conclusion:The expression of MID2 in gastriccancer tissues is upregulated.High MID2 expression is associated with a poorer prognosis in patients with gastric cancer.Upregulating the expression of MID2 can significantly promote the malignant phenotype of gastric cancer cells.