PDGF促进创面修复的研究进展

Research Progress of PDGF Promoting Wound Repair

血小板衍生生长因子(platelet-derived growth factor, PDGF)是一种多效能细胞因子,因具有启动细胞增殖、迁移、分泌并促进血管形成等作用,可促进伤口愈合,在创面修复领域备受关注。目前,含高浓度PDGF的富血小板血浆及重组人PDGF应用于创面修复已有数十年历史,因存在保质期短、异体使用受限或生物利用率低、副作用多等不足,使得其无法满足临床需求,而优化的富血小板纤维蛋白和浓缩生长因子,则具有更高的PDGF浓度,创面修复能力更佳。血小板裂解液保存期限可显著延长,且具有更小的异体使用风险,已成为血小板创面修复的发展方向。基于生物工程技术构建的高能递送系统可显著提高PDGF局部驻留率、延长作用时间,经过对PDGF结构进行改造,添加活性结构域可设计出多种能诱发持续强直信号的转化型PDGF,将活性结构域整合形成功能可控的PDGF模拟肽,并衍生了多种高效的PDGF基因递送策略,为解决创面修复问题提供了多种潜在选择。本文主要阐述PDGF的生理特性与应用特性及其在创面修复中的研究进展。

Platelet-derived growth factor(PDGF),a multi-potent cytokine,has caught attention in the field of wound repair.The PDGF ability to initiate cell proliferation,migration,secretion and promote angiogen-esis can be beneficial for wound healing.Platelet-rich plasma and recombinant human PDGF that contain high concentrations of PDGF have been used for decades in wound repair.However,their short shelf life,limited al-logenic use,low bioavailability,and many side effects make them unable to meet clinical needs.Optimized platelet-rich fibrin and concentrated growth factor have higher concentrations of PDGF and better wound repair ability.Platelet lysate,with the ability to significantly extend the preservation period and reduce the risk of allo-geneic use,has become the development direction for platelet wound repair.The high-energy delivery system based on bioengineering technology can significantly improve the local resident rate and prolong the action time of PDGF.A variety of transformed PDGF capable of inducing sustained tonic signals can be created through the modification of PDGF structure and addition of active domains.Functional controllable PDGF simulation pep-tides can be generated by integrating the active domains and a variety of efficient PDGF gene delivery strategies can be derived,thus providing more potential options to solve the problem of wound repair.The focus of this ar-ticle,is on reviewing the physiological and applied.