嗜酸乳杆菌M6提高小鼠运动能力和缓解疲劳功能的研究

Effect of Lactobacillus acidophilus M6 on Improving Exercise Performance and Relieving Fatigue in Mice

目的:前期从长跑运动员粪便中分离出嗜酸乳杆菌M6,本研究旨在研究嗜酸乳杆菌M6对提高小鼠运动能力和缓解疲劳功能的效果。方法:将60只C57BL/6N雄性小鼠随机分为4组:对照组、嗜酸乳杆菌M6低、中、高剂量组(依次为600、800及1000 mg/kg),每天灌胃嗜酸乳杆菌M6,连续灌胃4周,检测小鼠肌肉力量和力竭游泳时间,检测小鼠疲劳相关指标、能量相关指标和抗氧化相关指标。结果:低、中、高剂量的嗜酸乳杆菌M6均显著(P<0.001)提高小鼠前肢握力(124.01±6.02、132.02±4.30和139.66±4.72 g)和力竭游泳时间(8.03±1.05、10.06±1.10和14.36±0.55 min),均显著(P<0.001)降低小鼠血清肌酸激酶活力(730.66±16.77、647.66±39.95和594.56±32.33 U/L)、血乳酸(10.13±1.00、8.36±0.99和6.09±0.36 mol/L)、血尿素氮(6.40±0.53、5.96±0.85和5.23±0.25 nmol/L)和血氨(115.34±4.50、99.67±4.50和94.33±4.72μmol/L)等疲劳指标。此外,嗜酸乳杆菌M6显著(P<0.001)增加血糖、肌糖原和肝糖原,并显著(P<0.001)降低血清甘油三酯含量等能量代谢指标,显著(P<0.001)增加小鼠血清超氧化物歧化酶、过氧化氢酶及谷胱甘肽过氧化物酶的酶活,并显著(P<0.001)降低丙二醛含量。在3×107~3×109CFU嗜酸乳杆菌M6范围内存在剂量效应。结论:嗜酸乳杆菌M6可以通过提高小鼠前肢握力和力竭游泳时间改善小鼠运动能力的水平,通过降低小鼠肌酸激酶活力、乳酸、尿素氮和血氨,增加能量代谢,提高抗氧化能力,进而缓解运动后疲劳。

Objective:To isolate Lactobacillus acidophilus M6 from the feces of long-distance runners in the early stage.The aim of this study was to investigate the effect of Lactobacillus acidophilus M6 on improving exercise ability and alleviating fatigue function in mice.Methods:Sixty C57BL/6N male mice were randomly divided into 4 groups:Control,low,medium and high dose groups of L.acidophilus M6,L.acidophilus M6 was given daily for four weeks,muscle strength,exercise endurance,fatigue related indicators,energy related indicators and antioxidant related indicators were measured.Results:The low,medium and high dose of L.acidophilus M6 significantly(P<0.001)improved the forelimb grip strength(124.01±6.02,132.02±4.30 and 139.66±4.72 g)and exhaustive swimming time(8.03±1.05,10.06±1.10 and 14.36±0.55 min).The low,medium and high dose of L.acidophilus M6 significantly(P<0.001)reduced the fatigue indexes including the creatine kinase activity(730.66±16.77,647.66±39.95 and 594.56±32.33 U/L),serum lactic acid(10.13±1.00,8.36±0.99 and 6.09±0.36 mol/L),serum urea nitrogen(6.40±0.53,5.96±0.85 and 5.23±0.25 nmol/L)and blood ammonia(115.34±4.50,99.67±4.50 and 94.33±4.72μmol/L).L.acidophilus M6 significantly(P<0.001)improved energy metabolism indexes including increasing blood glucose,muscle glycogen and liver glycogen,and significantly(P<0.001)reducing triglyceride content in a dose-dependent manner.L.acidophilus M6 significantly(P<0.001)increased the activity of serum superoxide dismutase,catalase and glutathione peroxidase and decreased the content of malondialdehyde in mice in a dose-dependent manner at the range from 3×107 CFU to 3×109 CFU.Conclusion:L.acidophilus M6 could improve exercise performance and relieve fatigue after exercise in mice by improving the grip strength of forelimbs and the exhaustion swimming time,and increase the indexes related to energy metabolism and antioxidant capacity by decreasing the fatigue indexes such as creatine kinase activity,lactic acid,urea nitrogen and blood ammonia.