一种基于香豆素的荧光探针的合成及其对铝离子和锌离子的识别

Synthesis and properties of a coumarin-based fluorescent probe for the detection of Al~(3+) and Zn~(2+)

设计合成了以4-甲基-7-羟基-8-醛基香豆素为荧光团,4-氨基安替比林为识别位点的探针ACAP,通过红外光谱、核磁共振氢谱及碳谱对探针的结构进行了表征。利用荧光光谱研究了探针对不同金属离子的识别性能。结果显示,在CH_(3)OH/Tris-HCl(9∶1,V∶V)缓冲溶液中,探针ACAP对Al^(3+)和Zn^(2+)具有选择性识别及良好的识别灵敏性。离子竞争实验显示,其它共存离子对探针识别Al^(3+)/Zn^(2+)几乎没有影响,表明探针具有良好的抗干扰性。结合比测试显示,探针ACAP与Al^(3+)和Zn^(2+)均以1∶1结合。探针ACAP对Al^(3+)和Zn^(2+)的检出限分别为1.78μmol/L和0.194μmol/L,均低于世卫组织(WHO)对饮用水中Al^(3+)和Zn^(2+)所允许的最高浓度。

A probe ACAP was designed and synthesized using 4-methyl-7-hydroxy-8-uronic coumarin as fluorophore and 4-amino-antipyrine as recognition site.The structure of the probe was characterized by infrared spectrum,1H-NMR and 13C-NMR spectra.The recognition performance of the probe for different metal ions was investigated by fluorescence spectroscopy.The results showed that the probe ACAP had a selective recognition and a good sensitivity to Al^(3+) and Zn^(2+) in CH3OH/Tris-HCl(9:1,V:V) buffer solution.Ion competition experiments revealed that other co-existing ions had little effects on Al^(3+)/Zn^(2+) recognition,indicating that the probe possessed a good anti-interference.Binding ratio test manifested that the probe ACAP bond with Al^(3+)/Zn^(2+)in a 1∶1 manner.The detection limits for Al^(3+) and Zn^(2+) were estimated to be 1.78 μmol/L and 0.194 μmol/L,respectively,which were lower than the permissible concentrations of Al^(3+) and Zn^(2+) described by World Health Organization(WHO) in drinking water.