天冬氨酸修饰纳米金-纸芯片体系对高半胱氨酸的选择性检测

Selective determination of homocysteine by a paper-based assay system using aspartic acid modified gold nanoparticles

建立了以纸芯片为分析平台的高半胱氨酸(Hcy)快速检测新方法。Hcy使天冬氨酸(Asp)修饰的纳米金(Asp-AuNPs)体系发生团聚,溶液由红色逐渐变为蓝色。使用手机拍照记录结果,Photoshop软件分析显色强度。红/蓝通道颜色比值(ΔRed/Blue)与Hcy浓度在0.74~74μmol/L范围内呈良好线性关系,回归方程为y=0.0704x+0.5445,相关系数(R)为0.9995,检出限为0.37μmol/L,加标回收率为98.7%~100.3%。共存还原性物质干扰误差在±2%以内。该方法测得人血浆中Hcy含量为(5.52±0.39)μmol/L,符合人体血浆中Hcy浓度参考范围。

A novel method for homocysteine(Hcy) determination on paper-based analytical devices using aspartic acid(Asp) modified gold nanoparticles(Asp-AuNPs) was proposed.Hcy interacts with Asp-AuNPs selectively and leads to the aggregation of Asp-AuNPs after its addition.A color change from red to blue can be observed on the paper-based analytical devices.The results were recorded using a common cell phone and subsequently analyzed by Photoshop software.The ratio of color intensity at red and blue channel (ΔRed/Blue) increased linearly in the Hcy concentration range of 0.74-74 μmol/L,with the relative coefficient(R) of 0.9995.The regression equation was y=0.0704x+0.5445,the limit of detection was 0.37 μmol/L,and the recoveries of this method were 98.7%-100.3%.The interference deviations of coexisting reductive substances to Hcy were in the range of-2% to 2%.The Hcy concentration in human plasma was determined as(5.52±0.39) μmol/L by this method,which was consistent with the reference range of Hcy.