基于数据库挖掘分析 FN1、COL4A1、COL4A2基因在胃癌中的表达及意义

Analysis of FN1,COL4A1,COL4A2 genes expression and significance in gastric cancer based on database mining

目的基于数据库挖掘分析胃癌预后预测和胃癌靶向治疗的潜在关键基因(Hub基因)。方法本研究选取基因表达综合数据库(GEO)的GSE33651和GSE118916数据集(研究时间为2011年11月-2019年8月)。GEO2R进行胃癌差异表达基因(DEGs)分析。DAVID数据库对DEGs进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析。采用STRING数据库和Cytoscape软件构建DEGs的蛋白质-蛋白质相互作用(PPI)网络,其中Degree>10的DEGs被认为是Hub基因。基于癌症基因组图谱(TCGA)的胃腺癌数据,使用OncoLnc和UALCAN数据库对Hub基因进行生存分析和表达分析。TIMER数据库对Hub基因进行免疫浸润分析。结果GSE33651和GSE118916中鉴定出80个共有上调和34个共有下调DEGs。DEGs富集在69个GO条目和7个KEGG信号通路。从PPI网络中筛选出14个Hub基因。FN1、COL4A2和COL4A1基因在胃癌组织的表达水平均显著高于胃正常组织,且在胃癌中具有良好的预后预测价值。FN1、COL4A2和COL4A1与免疫浸润密切相关,提示其可能参与胃癌的免疫反应。结论FN1、COL4A2、COL4A1是胃癌的Hub基因,可能是潜在的胃癌预后生物学标志物和治疗靶点。

Objective To identify the potential hub genes for the prognosis and targeted therapy of stomach cancer.Methods GSE33651 and GSE118916 datasets were selected from gene expression omnibus(GEO,study periods were from November 2011 to August 2019).The differentially expressed genes(DEGs)were identified by GEO2R.Gene ontologty(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses of DEGs was performed in DAVID database.Protein-protein interaction(PPI)network was constructed using STRING database and Cytoscape software.DEGs with Degree>10 were considered as hub genes.Based on gastric adenocarcinoma data from the cancer genome atlas(TCGA),survival and expression analyses of hub genes were performed in OncoLnc and UALCAN databases,respectively.Immune infiltration analysis of hub genes was performed using TIMER database.Results A total of 80 up-regulated and 34 down-regulated DEGs were identified,enriched in 69 GO terms and 7 KEGG pathways.Fourteen hub genes were screened from the PPI network.The expression levels of FN1,COL4A2 and COL4A1 in gastric cancer tissue were higher than those in normal gastric tissues,and showed excellent prognostic values in gastric cancer.FN1,COL4A2 and COL4A1 were closely associated with immune cell infiltration,which might be involved in the immune response of gastric cancer.Conclusion FN1,COL4A2 and COL4A1 are the hub genes of gastric cancer,which may be potential prognostic biomarkers and therapeutic targets for gastric cancer.