直肠癌患者Ki-67、HER-2、p53表达与临床病理特征之间的关系

Relationship between Ki-67,HER-2 and P53 expression and clinicopathological features in patients with rectal cancer

目的探讨分析细胞核增殖抗原(Ki-67)、人类表皮生长因子受体-2(HER-2)和肿瘤抑制因子(p53)在直肠癌患者癌组织中的表达水平及其与临床病理特征之间的关系。方法选取2020年6月至2023年3月于本院肛肠科行手术治疗的直肠癌患者,收集癌组织标本及同一患者的癌旁正常组织标本各80例。应用免疫组化法检测Ki-67、HER-2和p53在直肠癌患者癌组织标本中的表达水平,分析其与临床病理学资料之间的关系,并应用Spearman分析3种基因表达之间的相关性。结果直肠癌患者癌组织标本中Ki-67、HER-2和p53表达阳性率均显著高于同一例患者的癌旁正常组织,差异存在统计学意义(P<0.05)。直肠癌患者癌组织标本中Ki-67、HER-2、p53表达阳性与TNM分期、组织学分型、脉管浸润及病理分化程度之间存在相关性(P<0.05);Ki-67和p53表达阳性与肿瘤直径之间存在相关性(P<0.05);Ki-67和HER-2还与淋巴结转移之间存在相关性(P<0.05),而三者均与性别之间无相关性(P>0.05)。直肠癌患者癌组织标本中Ki-67表达与HER-2和p53表达呈正相关(r=0.225、0.312,P<0.05);HER-2表达和p53表达也呈正相关(r=0.229,P<0.05)。结论Ki-67、HER-2和p53是直肠癌的有效诊断指标,Ki-67、HER-2和p53表达阳性与TNM分期、组织学分型、脉管浸润及病理分化程度之间相关,且三者之间均具有相关性。

Objective To investigate and analyze the expression levels of nuclear proliferation antigen(Ki-67),human epidermal growth factor receptor-2(HER-2)and tumor suppressor(p53)in cancer tissues of rectal cancer patients and their relationship with clinicopathological features.Methods Patients with rectal cancer who underwent surgery in the general surgery department of our hospital from June 2020 to March 2023 were included.80 cancer tissue specimens and 80 normal tissue specimens adjacent to cancer from the same patients were collected.The expression levels of Ki-67,HER-2 and p53 in rectal cancer specimens were measured by immunohistochemistry.The relationship between them and the clinicopathological data was analyzed.The correlation between the expression of the three genes was analyzed by Spearman.Results The positive expression rates of Ki-67,HER-2 and p53 in cancer specimens from rectal cancer patients were significantly higher than those in normal tissues adjacent to cancer in the same patients,and the differences were statistically significant(P<0.05).There was a correlation between positive expression of Ki-67,HER-2 and p53 in cancer tissue specimens from rectal cancer patients and TNM stage,histological staging,vascular infiltration and degree of pathological differentiation(P<0.05);a correlation between positive expression of Ki-67 and p53 and tumor diameter(P<0.05);and a correlation between Ki-67 and HER-2 and lymph node metastasis.There was also a correlation between Ki-67 and HER-2 and lymph node metastasis(P<0.05),while there was no correlation between all three and gender(P>0.05).Ki-67 expression was positively correlated with HER-2 and p53 expression in rectal cancer specimens(r=0.225、0.312,P<0.05);HER-2 expression was also positively correlated with p53 expression(r=0.229,P<0.05).Conclusion Ki-67,HER-2 and p53 are valid diagnostic indicators for rectal cancer.Positive Ki-67,HER-2 and p53 expression correlated with TNM stage,histological staging,vascular infiltration and degree of pathological differentiation,and all three correlated with each other.