子宫内膜癌组织MMR、NLRC3、FOXP1表达及与预后关系

Expressions of Mismatch repair protein,nucleotide binding oligomeric domain-like receptor protein 3,and forkhead frame protein P 1 in endometrial carcinoma tissues of patients and their correlation with the prognosis of the patients

目的:探讨子宫内膜癌组织中错配修复(MMR)蛋白、核苷酸结合寡聚化结构域样受体蛋白3(NLRC3)及叉头框蛋白P1(FOXP1)表达及与预后关系。方法:收集2018年1月-2021年1月本院收治的子宫内膜癌组织标本40例为病例组,非内膜癌组织40例为对照组。采用免疫组化染色分析两组MMR、NLRC3及FOXP1阳性表达率及与病例组预后关系。结果:病例组40例标本中,MMR蛋白总缺失率为32.5%,其中PMS2蛋白缺失1例(2.5%)、MSH6蛋白缺失2例(5.0%)、MLH1/PMS2蛋白同时缺失7例(17.5%)、MSH2/MSH6蛋白同时缺失3例(7.5%);NLRC3阳性表达率为22.5%,FOXP1阳性表达率为20.0%,均低于对照组(P<0.01);不同年龄、肌层浸润深度、组织学分级患者内膜癌组织中MMR、NLRC3及FOXP1表达无差异;肿瘤家族史、病理类型、临床分期、淋巴结转移、累及子宫下段患者的内膜癌组织中MMR、NLRC3及FOXP1阳性表达率有差异(P<0.05);死亡组MMR缺失量及NLRC3、FOXP1阳性表达率均低于存活组(P<0.05)。相关性分析显示,MMR、NLRC3、FOXP1与子宫内膜癌患者家族史、病理类型、临床分期、淋巴结转移、累及子宫下段及预后均呈负相关(均P<0.05)。结论:子宫内膜癌组织中MMR缺失量及NLRC3、FOXP1阳性表达率明显降低,可能协同参与了子宫内膜癌的发生发展,对患者预后有参考意义。

Objective:To explore the expressions of Mismatch repair(MMR)protein,nucleotide binding oligomeric domain-like receptor protein 3(NLRC3),and forkhead frame protein P 1(FOXP1)in endometrial carcinoma tissues of patients and their correlation with the prognosis of the patients.Methods:The endometrial carcinoma specimens of 40 patients with endometrial cancer(in study group)from January 2018 to January 2021 were collected,and the endometrial tissues of other 40 patients without endometrial cancer(in control group)were collected.Immunohistochemical staining was used to analyze the positive expression rates of MMR,NLRC3,and FOXP1 in the endometrial tissues of the patients in the two groups,and the correlation between the positive expression rates of MMR,NLRC3,and FOXP1 of the patients in the study group and their prognosis.Results:In the 40 specimens of the patients in the study group,the total deletion rate of MMR protein was 32.5%,including 1(2.5%)case with PMS2 protein deletion,2(5.0%)cases with MSH6 protein deletion,7(17.5%)cases with MLH1/PMS2 protein deletion,and 3(7.5%)cases with MSH2/MSH6 protein deletion.The positive expression rates of NLRC3 and FOXP1 of the patients in the study group were 22.5% and 20.0%,both of which were significantly lower than those of the patients in the control group(P<0.01).There were no significant differences in the expressions of MMR,NLRC3,and FOXP1 in the endometrial carcinoma tissues among the patients with different age,among the patients with different depth of muscle infiltration,and among the patients with different histological grade.There were significant differences in the positive expression rates of MMR,NLRC3,and FOXP1in endometrial carcinoma tissues among the patients with different tumor family history,among the patients with different pathological type,among the patients with different clinical stage,among the patients with different lymph node metastasis,and between the patients with and without endometrial carcinoma involved their lower uterine segment(P<0.05).The amount of MMR deletion and the positive expression rates of NLRC3and FOXP1of the deceased were significantly lower than those of the survivors(P<0.05).The correlation analysis showed that the expressions of MMR,NLRC3,and FOXP1of the patients with endometrial carcinoma were negatively correlated with their family history,pathological type,clinical stage,lymph node metastasis,involvement of lower uterine segment,and prognosis(all P<0.05).Conclusion:The amount of MMR deletion and the positive expression rates of NLRC3and FOXP1in the endometrial cancer tissues are reduced significantly,which may be synergistically involved in the occurrence and development of the endometrial cancer and has some reference significance for the prognosis of the patients.