荧光原位杂交和NanoString nCounter技术检测乳腺癌HER2的表达及其与化疗疗效的相关性

Comparison of NanoString nCounter and FISH data on HER2 Expression in FFPE BC Samples

目的探讨荧光原位杂交和NanoString nCounter技术检测HER2蛋白在乳腺癌FFPE组织样本中的表达及对化疗疗效的影响。方法分别采用荧光原位杂交和NanoString nCounter技术检测53例乳腺癌及癌旁组织中HER2蛋白的表达,对两种方法的检测结果进行对比,检测结果分别和蒽环类化疗药物的疗效进行对比。结果FISH检测53例乳腺癌FFPE组织标本中HER2阳性率为56.60%(30/53);NanoString nCounter技术检测HER2阳性率为69.81%(37/53);将FISH结果作为金标准,NanoString nCounter检测敏感度为93.32%,特异性为60.87%。FISH检测HER2阳性的30例患者中,19例化疗有效(63.33%)(CR+PR),11例化疗无效(36.67%)(SD+PD);23例阴性结果中,10例化疗有效(43.48%),13例化疗无效(56.52%)(P=0.412)。NanoString nCounter技术检测HER2阳性的37例患者中,19例化疗有效(51.35%),18例化疗无效(48.65%);16例阴性患者中,3例化疗有效(18.75%),13例化疗无效(81.25%)(P=0.039)。结论与FISH方法相比,NanoString nCounter技术结果有较高的敏感度和特异性,且HER2的NanoString nCounter技术的检测结果和化疗疗效更相关。

Objective To compare the results of fluorescence in situ hybridization(FISH)and NanoString nCounter in detecting the expression of HER2 protein in FFPE tissue samples of breast cancer,and evaluate the performance of NanoString nCounter’s new technology.Methods FISH and NanoString nCounter were used to detect the expression of HER2 protein in 53 pairs of BC tissues and adjacent tissues respectively.The detection results of the two methods were compared.Furthermore,the results were compared with the efficacy of anthracycline chemotherapy.Results The positive HER2 and the negative HER2 were 56.60%(30/53)in FFPE tissue samples of 53 cases of BC by FISH,while 69.81%(37/53)by NanoString nCounter.Taking FISH results as the gold standard,NanoString nCounter’s detection sensitivity was 93.32%and specificity was 60.87%.Among the 30 patients with positive HER2 detected by FISH,chemotherapy was effective(CR+PR)in 19 patients(63.33%),ineffective(SD+PD)in 11 patients(36.67%);while the 23 patients with the negative HER2 results,chemotherapy was effective in 10 patients(43.48%)and ineffective in 13 patients(56.52%)(P=0.412).NanoString nCounter technology detected that chemotherapy was effective in 19 patients(51.35%)while ineffective in 18 patients(48.65%),with positive HER2;effective in 3 patients(18.75%)and ineffective in 13 patients(81.25%)with negative HER2,(P=0.039).Conclusion Compared with FISH method,NanoString nCounter technology has a higher sensitivity and specificity,and the detection results of HER2 NanoString nCounter technology are more related to the efficacy of chemotherapy.