海苔生产用紫菜菌落总数分析及其优势菌鉴别

Analysis of Total Bacterial Count and Identification of Dominant Bacteria in Seaweed Production Using Seaweed

通过菌落总数分析及对其优势菌进行鉴别,评价紫菜带菌情况。采用平板计数、生理生化实验和16S rDNA测序技术,对海苔生产用紫菜进行菌落总数及优势菌分析。结果显示,2020—2022年紫菜菌落总数在1×10^(3)~5×10^(7)CFU·g^(-1)。从紫菜中分离出4个优势菌,通过生理生化培养及16S rDNA测序确定为深海微小杆菌(Exiguobacterium profundum)、印度考克氏菌(Kocuria indica)、解多聚物奇异球菌(Deinococcus depolymerans)、枯草芽孢杆菌(Bacillus subtilis)。本次研究发现,紫菜中菌落总数没有规律性,含菌量高低不一,其含有的枯草芽孢杆菌对干热、湿热、辐照、干燥、氧化以及强酸等不利条件均具有很高的耐受性,为海苔生产企业制定生产工艺提供了有力的支撑。

Evaluate the carrier status of seaweed by analyzing the total bacterial count and identifying its dominant bacteria.Using plate counting,physiological and biochemical experiments,and 16S rDNA sequencing technology,the total bacterial count and dominant bacteria of seaweed used for seaweed production were analyzed.The results show that the total number of seaweed colonies in 2020—2022 years has been 1×10^(3)~5×10^(7) CFU·g^(-1).Four dominant bacteria were isolated from seaweed and identified through physiological and biochemical cultivation and 16S rDNA sequencing as Exiguobacterium profundaum,Kocuria index,Deinococus dimers,and Bacillus subtilis.This study found that there is no regularity in the total number of bacterial colonies in seaweed,and the bacterial content varies.The Bacillus subtilis it contains has high tolerance to adverse conditions such as dry heat,humid heat,irradiation,drying,oxidation,and strong acid,providing strong support for seaweed production enterprises to develop production processes.