纳米氧化铈对阿霉素诱导的心脏损伤的影响

Effect of Cerium Oxide Nanoparticles on Adriamycin-induced Cardiac Injury

目的:分析纳米氧化铈(CeNPs)对阿霉素(Dox)诱导的心脏损伤的影响。方法:培养H9C2大鼠心肌细胞,Dox处理建立细胞损伤模型,根据随机数字表法分为空白对照组、Dox组、CeNPs组、CeNPs+Dox组、右丙亚胺(DEX)+Dox组,各5例。CCK-8法测定心肌细胞活力,蛋白质印迹法测定各组B淋巴细胞瘤-2(Bcl-2)、Bax的蛋白表达量,生化法检测相关指标胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果:与空白对照组比较,Dox组心肌细胞活力降低;与Dox组比较,CeNPs组、CeNPs+Dox组、DEX+Dox组心肌细胞活力增强,差异有统计学意义(P<0.05)。与空白对照组比较,Dox组Bax蛋白表达水平增加,Bcl-2蛋白表达水平下降,差异有统计学意义(P<0.05);与Dox组比较,CeNPs组、CeNPs+Dox组、DEX+Dox组Bax蛋白表达水平下降,Bcl-2蛋白表达水平增高,差异有统计学意义(P<0.05)。与空白对照组比较,Dox组GPx活力下降,MDA含量上升,差异有统计学意义(P<0.05);与Dox组比较,CeNPs组、CeNPs+Dox组、DEX+Dox组GPx活力升高,MDA含量下降,差异有统计学意义(P<0.05)。结论:CeNPs对Dox诱导的H9C2心肌细胞具有保护作用,其作用机制可能与抗氧化应激、改善细胞凋亡蛋白相关。

Objective:To analyze the effects of cerium oxide nanoparticles(CeNPs)on adriamycin(Dox)-induced cardiac injury.Methods:H9C2 rat cardiomyocytes were cultured,and Dox treatment was used to establish a cell injury model,which was divided into blank control,Dox,CeNPs,CeNPs+Dox,and dextrazoxane(DEX)+Dox groups according to randomized numerical table method,with five cases each.CCK-8 method was used to determine the viability of cardiomyocytes,protein expression of B lym-phocytoma-2(Bcl-2)and Bax in each group was determined by protein blotting,and relevant index cysteine peroxidase(GSH-Px)activity and malondialdehyde(MDA)content were detected by biochemical methods.Results:Compared with the blank control group,cardiomyocyte viability was reduced in the Dox group,and compared with the Dox group,cardiomyocyte viability was en-hanced in the CeNPs group,the CeNPs+Dox group,and the DEX+Dox group,and the difference was statistically significant(P<0.05).Compared with the blank control group,Bax protein expression level increased and Bcl-2 protein expression level decreased in the Dox group,and the difference was statistically significant(P<0.05);Compared with Dox group,Bax protein expression level de-creased and Bcl-2 protein expression level increased in CeNPs group,CeNPs+Dox group and DEX+Dox group,and the difference was statistically significant(P<0.05).Compared with the blank control group,GPx activity decreased and MDA content increased in the Dox group,and the difference was statistically significant(P<0.05);Compared with the Dox group,GPx vitality increased and MDA content decreased in the CeNPs group,CeNPs+Dox group and DEX+Dox group,and the difference was statistically sig-nificant(P<0.05).Conclusion:CeNPs had a protective effect on Dox-induced H9C2 cardiomyocytes,and its mechanism of action might be related to anti-oxidative stress and improvement of apoptotic proteins.