2020—2021年宁夏地区牛支原体血清流行病学调查与分析

Seroepidemiological investigation and analysis of Mycoplasma bovis in Ningxia region from 2020 to 2021

为了初步掌握宁夏地区牛支原体(Mycoplasma bovis,M.bovis)病的流行情况,试验采用ELISA方法对2020—2021年宁夏地区5个城市22个县/区收集的牛血清样本进行牛支原体血清抗体检测,分析不同年份、不同城市、不同县/区血清学调查结果。结果表明:宁夏地区5个城市固原市、石嘴山市、吴忠市、中卫市和银川市牛支原体抗体的检测均呈阳性。2020年和2021年平均牛支原体抗体阳性率分别为(29.39±15.07)%、(37.58±19.52)%,2021年与2020年相比,血清牛支原体抗体阳性率差异极显著(P<0.01);不同城市间比较,固原市、中卫市、石嘴山市、吴忠市和银川市两年平均牛支原体抗体阳性率分别为(22.00±8.78)%、(35.56±14.09)%、(31.11±10.47)%、(50.00±25.91)%、(29.44±9.19)%,其中吴忠市平均牛支原体抗体阳性率最高,固原市最低;不同县/区间比较,西吉县和同心县两年的牛支原体抗体阳性率未发生变化,2021年原州区和平罗县的牛支原体抗体阳性率与2020年相比下降,其他18个县区2021年的牛支原体抗体阳性率与2020年相比均上升,其中利通区和红寺堡区两年比较差异显著(P<0.05)。说明2020—2021年宁夏地区牛支原体血清抗体阳性率呈上升趋势。

In order to initially grasp the prevalence of Mycoplasma bovis(M.bovis)disease in Ningxia region,the experiment was conducted by using ELISA method to detect M.bovis serum antibodies in bovine serum samples collected from 22 counties/districts in 5 cities in Ningxia region from 20202021 and to analyze the results of serological investigation in different years,cities and counties/districts.The results showed that M.bovis antibody detection was all positive in five cities in Ningxia region,Gouyuan City,Shizuishan City,Wuzhong City,Zhongwei City and Yinchuan City.The average M.bovis antibody positivity rates in 2020 and 2021 were(29.39±15.07)%,(37.58±19.52)%,respectively.Highly significant difference was in positive rates of serum M.bouis antibody in 2021 compared to 2020(P<0.01).Comparing between different cities,the two-year average M.bovis antibody positive rates were(22.00±8.78)%,(35.56±14.09)%,(31.11±10.47)%,(50.00±25.91)%,and(29.44±9.19)%in Guyuan City,Zhongwei City,Shizuishan City,Wuzhong City,and Yinchuan City respectively;the highest average M.bovis antibody positive rate was in Wuzhong City,and the lowest in Guyuan City.The positive rates of M.bovis antibody in Xiji County and Tongxin County did not change in two years when compared among different counties/districts.The positive rates of M.bovis antibody in Yuanzhou District and Pingluo County in 2021 decreased compared to the positive rates in 2020.The positiverates of M.bovis antibody in the other18 counties and districts in 2021 showed an increasing trend compared with 2020,witha significant difference(P<0.05)between the two years of comparison in Litong and Hongsibao Districts.The results indicated that the positive rates of serum M.bovis antibody had an increasing trend in Ningxia region over a 2-year period.