类普鲁士蓝纳米酶材料的制备及其酶催化反应动力学探究——推荐一个分析化学综合实验

Preparation of Prussian Blue Analogs Derived Nanoenzymes and Their Enzymatic Catalytic Reaction Kinetics:A Comprehensive Analytical Chemical Experiment

将类普鲁士蓝纳米酶引入本科实验教学,设计了一个探究性的分析化学综合实验,实验内容包括三种类普鲁士蓝纳米酶材料的制备、表征和酶催化反应速率常数的测定,并将这些纳米酶材料应用于过氧化氢(H2O2)的分析检测。本实验所用试剂价廉易得、反应条件温和、实验现象直观、时间分配合理,适合本科实验教学,用于提升学生的实验技能和科研创新能力。

An exploratory analytical chemistry comprehensive experiment was designed based on Prussian blue analog-derived nanoenzymes.The experiment includes the preparation and characterization of three kinds of Prussian blue analog-derived nanoenzyme materials and the determination of their enzyme catalytic reaction rate constants.These nanomaterials were further applied to hydrogen peroxide detection.As this experiment features inexpensive reagents,mild reaction conditions,obvious experimental phenomena,and reasonable time allocation,it is suitable for undergraduate laboratory teaching to improve students’experimental skills and cultivate their scientific research and innovation ability.