鹿角菜醇提物不同极性部位活性成分及其体外抗氧化活性研究

Analysis of active components in different polar parts of Pelvetia siliquosa ethanol extract and their antioxidant activity in vitro

研究鹿角菜醇提物不同极性部位总黄酮、总多酚和总三萜质量浓度及其抗氧化活性关系。以鹿角菜为主要原料,采用标准曲线法计算鹿角菜醇提物各不同萃取相中总黄酮、总多酚和总三萜的含量,同时通过ABTS^(+)·法、DPPH·法、O_(2)^(-)·法和·OH法以及Fe^(3+)还原法,计算不同萃取相下的鹿角菜醇提物体外抗氧化能力。结果表明,鹿角菜醇提物中黄酮类物质含量最高,三萜类物质含量最低。通过不同极性部位比较发现,鹿角菜醇提物乙酸乙酯相中抗氧化活性物质总含量最高,抗氧化能力最强,其对ABTS^(+)·、DPPH·、O_(2)^(-)·及·OH的清除率及Fe^(3+)的还原性分别为98.17%、96.89%、31.99%、100%和1.1297。由此可见,鹿角菜醇提物的乙酸乙酯相可作为鹿角菜后期研究的核心。

In order to study the relationship between the mass concentration of total flavonoids,total polyphenols and total triterpenes in different polar parts of Pelvetia siliquosa alcohol extracts and their antioxidant activities,the content of total flavonoids,total polyphenols,and total triterpenes in different extraction phases of Pelvetia siliquosa alcohol extracts was calculated with standard curve method.The in vitro antioxidant capacity of the alcohol extracts of Pelvetia siliquosa from different extraction phases was measured by ABTS^(+)·method,DPPH·method,O_(2)^(-)·method,·OH method and Fe^(3+)reduction.The results showed that the highest ingredients in the alcohol extracts of Pelvetia siliquosa was flavonoids and the lowest was triterpenoids.Through the comparison of active components in different polar parts of the alcohol extracts of Pelvetia siliquosa,it was found that ethyl acetate phase of the alcohol extract of Pelvetia siliquosa had the highest total content of active components and had the strongest antioxidant capacity.Its scavenging rate of ABTS^(+)·,DPPH·,O_(2)^(-)·,and·OH and the reducibility of Fe^(3+)were 98.17%,96.89%,31.99%,100%and 1.1297,respectively.Future research can focus on the ethyl acetate phase of the alcohol extracts from Pelvetia siliquosa.