红小豆多酚对小鼠视网膜光损伤的保护作用及其机制研究

Effect of red bean polyphenols on protection of retina damage in mice and its protection mechanism

研究红小豆多酚对光诱导的视网膜损伤的保护作用及其作用机制。采用红小豆多酚连续8周灌胃小鼠并强光照射以建立小鼠视网膜光损伤模型,利用视网膜电图和光学相干断层成像对视网膜生理指标进行检测,并测定视网膜组织的抗氧化能力和炎症相关指标含量。结果表明:红小豆干预小鼠后视网膜b波振幅和ONL厚度均显著高于模型组(P<0.01)。红小豆多酚通过增加SOD、CAT和GSH-px酶活性提高T-AOC活性以降低MDA含量。红小豆多酚抑制炎症因子IL-1β、TNF-α、MCP-1和NO的释放,还可降低VEGF含量以避免视网膜组织血管增生来降低细胞炎性浸润。红小豆多酚通过调节Nrf2-HO-1途径调控抗氧化能力,下调氧化应激反应,减轻NF-κB途径参与的炎症反应来保护视网膜。红小豆多酚对光诱导的视网膜损伤具有保护作用。

The effects of red bean polyphenols on anti-inflammation and antioxidant capacity of light-induced retina damage mice were studied.After mice were gavaged with red bean polyphenols for 8 weeks,and with strong light irradiation on mice to establish the retinal damaged mice model.The physiological indexes of the retina were detected by electroretinogram(ERG)and optical coherence tomography(OCT),and the antioxidant capacity of retinal tissue and the content of inflammation related indexes were measured.The results showed that the amplitude of retinal b wave and the thickness of ONL in mice treated with red bean were significantly higher than those in the model group(P<0.01).Red bean polyphenols could increase T-AOC activity and reduce MDA content by increasing SOD,CAT and GSH-px enzyme activities.Red bean polyphenols could inhibit inflammatory factor IL-1β,TNF-α,MCP-1 and NO,it could also reduce VEGF content to avoid retinal vascular hyperplasia and reduce cellular inflammatory infiltration.The mechanism of red bean polyphenols inhibiting retinal light-induced damage was to regulate the antioxidant capacity by regulating Nrf2-HO-1 pathway,down regulating oxidative stress response,and reducing NF-κB pathway involved in the inflammatory reaction.Red bean polyphenols had protective effects on light-induced retinal injury in mice.