海口市湿地公园褐云玛瑙螺广州管圆线虫感染情况调查

Investigation on Angiostrongylus cantonensis infection in Achatina fulica in Haikou wetland park

为了解海口市湿地公园褐云玛瑙螺感染广州管圆线虫情况,分别于2021、2022年3—7月采集海口市某湿地公园两岸的公共绿地和乱石下的螺类并鉴定螺种,鉴定为褐云玛瑙螺的螺进行压片后镜检肺囊,“肺检法”检测褐云玛瑙螺的广州管圆线虫感染情况。提取阳性螺广州管圆线虫基因组DNA进行PCR扩增核糖体大亚基rRNA基因,测序后进行BLAST比对。2021—2022年从调查点共采集到褐云玛瑙螺207只,其中23只广州管圆线虫阳性,阳性率为11.11%(23/207)。PCR扩增结果显示在约400 bp处有特异性条带,符合预期大小。BLAST比对结果显示,PCR扩增产物测序结果与NCBI基因库中广州管圆线虫基因序列(GenBank序列号:AY295821.1、FM207759.1、AM039758.1和ON747308.1)的一致性为98.49%~99.75%。结果提示该湿地公园存在广州管圆线虫阳性的褐云玛瑙螺,接触、进食生或半生的螺肉存在较高的感染风险。

To investigate the prevalence of Angiostrongylus cantonensis infection in Achatina fulica in a wet⁃land park of Haikou,A.fulica were randomly collected from a public green land and underneath the ripraps of the wet⁃land park during March to July of 2021 and 2022.A.cantonensis infection among the A.fulica was determined by lung examination.A.cantonensis rRNA gene was amplified by PCR and the sequence was compared by BLAST analysis.The result suggested 23 of 207(11.11%)A.fulica were infected with A.cantonensis.The amplified 400 bp⁃DNA se⁃quence was close to the reference sequences of A.cantonensis in the NCBI GenBank(Accession number:AY295821.1,FM207759.1,AM039758.1 and ON747308.1).The sequence similarity ranged from 98.49%to 99.75%.In conclusion,A.cantonensis were found in A.fulica collected from the wetland park of Haikou.This will be provide a infection risk upon touching or eating the raw or partially cooked A.fulica.