五味子醇提物喷雾干燥工艺优化及体外抗氧化活性评价

Optimization of Spray Drying Process of Schisandra chinensis Alcohol Extract and Evaluation of its Antioxidant Activity in Vitro

基于单因素试验及响应面法优化五味子醇提物喷雾干燥工艺,采用体外抗氧化方法评价工艺优化前后喷干粉的体外抗氧化能力,为五味子醇提物的生产、加工提供一定实践及理论参考。以五味子醇甲含量及乙醇残留量为响应值,基于单因素试验及Box-benhnken响应面法优化试验研究不同进/出口温度、进料速度及雾化器离心频率对喷雾干燥工艺的影响;对所得的五味子醇提物进行体外抗氧化试验,评价抗氧化能力。结果表明,得到最优喷雾干燥工艺条件为进口温度160℃,出口温度85℃,进料速度3 mL/s,雾化器离心频率160 Hz,在此条件下五味子醇甲含量为0.85 g/100 g,乙醇残留量为0.34 g/100 g,经对比,工艺优化前后其五味子醇甲含量提升67%,乙醇残留量降低72%。体外抗氧化试验显示工艺改进后喷干粉对DPPH(2,2,-diphenyl-1-picrylhydrazyl)、ABTS(2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid))、CuPRAC(Cupric Reducing Antioxidant Power)及FRAP(Ferric Reducing Antioxidant Power)的清除能力均有所增强。通过优化喷雾干燥工艺中的进/出口温度、进料速度及雾化器离心频率可以进一步提升喷干粉中五味子醇甲含量并降低乙醇残留量,使其具有更高的体外抗氧化作用,即可为五味子醇提物生产及开发提供一定实践参考。

To optimize the spray drying process of Schisandra chinensis alcohol extract based on single factor and response surface methodology,and to evaluate the in vitro antioxidant capacity of the spray dried powder before and after the optimization of the process using the in vitro antioxidant method,so as to provide some practical and theoretical reference for the production and processing of Schisandra chinensis alcohol extract.Taking Schisandra chinensis schisandrin A content and ethanol residue as response values,the effects of different inlet/outlet temperatures,feed speeds and atomizer centrifugal frequency on spray drying process were studied based on single factor and Box Benhnken response surface method.The antioxidant capacity of the alcohol extract of Schisandra chinensis was evaluated by antioxidant test in vitro.The optimal spray drying process conditions were obtained as follows:the inlet temperature was 160℃,the outlet temperature was 85℃,the feed rate was 3 mL/s,and the atomizer centrifugal frequency was 160 Hz.Under this conditions,the schisandrin content was 0.85 g/100 g,and the ethanol residue was 0.34 g/100 g.After comparison,the schisandrin content increased by 67%before and after the process optimization,and the ethanol residue decreased by 72%.In vitro antioxidant test showed that the cleaning ability of spray dry powder after the process improvement on DPPH(2,2,-diphenyl-1-picrylhydrazyl),ABTS(2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)),CuPRAC(Copper Reducing Antioxidant Power)and FRAP(Ferric Reducing Antioxidant Power)was enhanced.By optimizing the inlet/outlet temperature,feeding speed and atomizer centrifugal frequency in the spray drying process,the content of schisandrin A in spray dried powder could be further increased,the residual amount of ethanol could be reduced and it had a higher antioxidant effect in vitro,which could provide some practical reference for the production and development of schisandrin alcohol extract.