中药材中真菌毒素免疫检测技术的研究进展

esearch progress in immunoassay of mycotoxins in traditional Chinese medicine

目的真菌毒素残留是引起中药材及其制品品质变化和用药安全的重要影响因素之一,为加强和提升中药材质量控制,需加强对中药材中的真菌毒素的检测。探讨中药材中真菌毒素免疫检测技术,为该领域的方法研究提供参考。方法通过查阅国内外文献,总结梳理了不同类型免疫检测技术在中药材真菌毒素检测中的应用。结果中药材中真菌毒素的免疫检测技术主要以免疫层析技术与酶联免疫吸附检测技术居多,荧光偏振免疫分析法、生物条形码免疫检测技术、电致化学发光免疫传感器、流式微球技术更多地处于科研阶段,产业化不多。结论随着快速免疫检测技术的研究和发展,将会对未来中药材真菌毒素快速检验和筛查提供广阔的发展空间。

Objective Mycotoxin residue is one of the important factors that bring changes in the quality and safety of Chinese medicinal materials and their products.It is necessary to strengthen the detection of mycotoxin in Chinese medicinal materials for strengthening and improving the quality control of Chinese medicinal materials.In order to provide reference for the method research in this field,immunoassay technology for mycotoxin in Chinese medicinal materials was discussed.Methods By reading domestic and foreign literature,the application of different types of immunoassay in the detection of mycotoxins in Chinese medicinal materials was presented and summarized.Results The immunoassay for mycotoxins in Chinese medicinal materials is mainly based on immunochromatography and enzyme-linked immunosorbent technology.Fluorescence polarization immunoassay,Bio-barcode assay,Electrogenerated chemiluminescence assay and cytometric bead array were all in research stage,there will be longtime to large-scale application.Conclusion With the development of rapid immunoassay technology,it will be a good prospect for rapid detection and screening of mycotoxins in Chinese medicinal materials in the future.