盐度和pH对平凡舟形藻生长和油脂积累的影响

SALINITY AND PH ON THE GROWTH AND TOTAL LIPID CONTENT OF NAVICULA TRIVIALIS

为研究盐度和pH对淡水硅藻生长和油脂含量的影响,对一株分离自野外采集水样中的平凡舟形藻(Navicula trivialis)进行研究,通过设置不同盐度0、0.03、0.06、0.12、0.18和0.24 mol/L和不同pH 4.5、6.0、7.5、8.5、9.5和10.5进行胁迫,测定各处理对平凡舟形藻的生长、叶绿素a含量、叶绿素荧光参数和油脂含量的影响。结果表明:盐浓度为0.12 mol/L、pH 7.5时,平凡舟形藻细胞密度和叶绿素a含量最高;盐浓度为0.24 mol/L、pH 7.5时,总脂含量最高,分别为34.93%和33.5%。结果表明,平凡舟形藻对不同盐度和pH的适应性不同,这在一定程度上影响其生长和油脂含量。

In order to study the effects of salinity and pH on the growth and lipid content of freshwater diatoms,we studied a strain of Navicula trivialis from field water samples,by setting different salinity 0,0.03,0.06,0.12,0.18,0.24 mol/L and pH 4.5,6.0,7.5,8.5,9.5 and 10.5 concentration stress.The effects of each treatment on the growth,chlorophyⅡa content,chlorophyⅡfluorescence parameters and lipid content of Navicula trivialis were determined.The results showed that the cell density and chlorophyⅡa content of Navicula trivialis were the highest when the NaCl concentration was 0.12 mol/L and pH 7.5;When NaCl concentration was 0.24 mol/L and pH 7.5,the total lipid content was the highest,which was 34.93%and 33.5%,respectively.The results showed that the adaptability of Navicula trivialis to different salinity and pH was different,which affected its growth and lipid content to some extent.Finding the best culture conditions to improve the growth and total lipid content of Navicula trivialis provides basic data for the study of microalgae culture,and the regulation of salinity and pH can provide scientific basis for the large-scale culture of Navicula trivialis.At the same time,the sensitivity,response and adaptability of Navicula trivialis to the salinity and pH,which will affect the phytoplankton community under environmental changes to a certain extent.the study provides a new idea for in-depth discussion of the impact of salinity and pH changes on phytoplankton community structure.