Golgi-Cox法标记小鼠脑神经方法的比较

Comparison of Golgi-Cox Method For Labeling Mouse Brain Nerves

为研究脑神经的结构特征,基于Golgi-Cox染色原理,比较了4种小鼠脑组织样品制备方法,探索了一种简单有效的神经标记方法。将小鼠断颈或麻醉后,采用4种不同的灌注方法,将取出的脑组织固定、浸染、振动切片、脱水及封片后进行光学显微镜成像,观察脑切片制备效果。比较这4种样品制备方法的光学显微镜成像结果,表明通过应用多聚甲醛固定的Golgi-Cox法制备的切片背景浅淡,非特异性沉淀颗粒少,且切片不易破碎,更加稳定。脑神经标记流程的探究为教学和科研提供了可选方案。

In order to study the structural characteristics of cranial nerves,four mouse brain tissue sample preparation methods are compared based on the principle of Golgi-Cox staining,and a simple and effective neural labeling method is explored.The preparation effect of brain slices is observed by first using different perfusion methods after neck breaking or anesthesia,and then by fixation,impregnation,vibration sectioning,dehydration transparency and sealing,the brain tissues are extracted.According to the light microscopy imaging results of the four sample preparation methods,the sections prepared by the Golgi-Cox method fixed by paraformaldehyde have a light background,few non-specific precipitated particles,and the sections are not easy to break and are more stable.Through the exploration of a set of brain neural labeling processes,it also provides support for teaching and scientific research.