糖尿病大鼠肾皮质糖基化终产物含量与纤溶酶原激活物抑制物-1表达的关系

Relationship between advanced glycation end product content and plasminogen activator inhibitor-1 expression in renal cortex of diabetic rats

目的 :研究链脲佐菌素诱发的糖尿病 ( DM)大鼠肾皮质糖基化终产物 ( AGEs)含量与纤溶酶原激活物抑制物 - 1 ( PAI- 1 )表达的关系及牛磺酸的调节作用。方法 :DM大鼠用牛磺酸处理 1 4周后 ,分别用荧光法、生物化学法和 Northern杂交检测肾皮质 AGEs含量、组织型纤溶酶原激活物( t- PA)活性、PAI- 1活性和 PAI- 1 m RNA表达。结果 :与对照大鼠相比 ,DM大鼠肾皮质 AGEs含量、PAI- 1活性和 PAI- 1 m RNA表达明显增加 ,而 t- PA活性下降。AGEs含量与 PAI- 1生物活性及 PAI- 1 m RNA表达均呈正相关 ( P<0 .0 1 )。DM大鼠给予牛磺酸处理后可改善上述参数的改变。结论 :DM大鼠肾皮质 AGEs含量增加能明显上调 PAI- 1基因表达 ,牛磺酸可抑制 AGEs形成和PAI- 1基因表达的上调。

Objective:To investigate the relationship between the advanced glycation end products(AGEs) content and plasminogen activator inhibitor 1 (PAI 1) gene expression in renal cortex of streptozotocin induced diabetic rats and its modulation by taurine. Methods:The fluorescence method, biochemical technique and Northern blot analysis were used to detect the AGE content, tissue plasminogen activator(t PA)activity,PAI 1 activity and PAI 1 mRNA expression, respectively. Results:Compared with control animals, diabetic rats showed an increase in AGE content,PAI 1 activity and PAI 1 mRNA expression,and a decrease in t PA activity in renal cortex. AGE content was positively correlated with PAI 1 activity and PAI 1mRNA expression. Administration of taurine to diabetic rats improved the changes of these parameters. Conclusion:The increased AGE content in renal cortex of diabetic rats can markedly upregulate PAI 1 mRNA expression, and taurine suppresses AGEs formation and PAI 1 mRNA expression.