基于数据挖掘的骨肉瘤动物模型应用分析

Application characteristics of animal models of osteosarcoma based on datamining

目的总结骨肉瘤(Osteosarcoma,OS)动物模型造模要点,为完善其造模方法和评价指标提供参考与建议。方法通过检索中国知网、万方数据库、PubMed中OS动物模型相关文献,总结其实验动物种类、性别、造模方法、癌细胞株种类、检测指标等,建立数据库进行统计分析。结果共纳入284篇文献,统计分析发现OS模型动物首选BALB/c-nu/nu裸鼠(227例,75.17%),其次是SD大鼠(20例,6.62%)。造模方式多选用背部皮下细胞液移植法(66例,21.85%)、腋部皮下细胞液移植法(55例,18.21%)或原位细胞液移植法(51例,16.89%)等;癌细胞株种类以人源MG-63细胞(100例,33.11%)、鼠源UMR-106细胞(39例,12.91%)为主;检测指标主要选择肿瘤组织表观指标(238例,83.80%)、肿瘤组织HE染色(129例,45.42%)、动物表观指标(94例,33.10%)、肿瘤组织免疫组化(89例,31.34%)。结论目前OS模型多选用4~6周龄BALB/c-nu/nu裸鼠作为实验动物,采用人源MG-63细胞异位移植法(背部、腋下移植)建立OS动物模型,模型检测指标以动物表观指标、肿瘤表观指标、肿瘤组织病理进行整体评价。但目前依旧缺少与临床吻合度高的动物模型制备及评价标准,本文通过文献挖掘、数据分析总结其优缺点,以期为建立良好的OS模型提供参考,更好地应用于OS机制研究及新药开发。

ObjectiveTo summarize the important points of animal models of osteosarcoma and to provide a reference and suggestions to improve the modeling method and evaluation indexes.MethodsUsing osteosarcoma and animals as the main topics,literature on animal models of osteosarcoma from CNKI,Wanfang Data and PubMed.The species of experimental animals,gender,modeling method,types of cancer cell lines and detection indicators were summarized,and a database was established for statistical analysis.ResultsA total of 284 reports were included.The most selected osteosarcoma model animals were BALB/c-nu/nu mice(227 times,75.17%),followed by SD rats(20 times,6.62%).Subcutaneous cell fluid transplantation in the back(66 times,21.85%),subcutaneous cell fluid transplantation in the axils(55 times,18.21%),and in situ cell fluid transplantation(51 times,16.89%)were used as modeling method.Human MG-63 cells(100 times,33.11%)and mouse UMR-106 cells(39 times,12.91%)were selected as the cancer cell line.The most analyzed indexes were the tumor tissue apparent index(238 times,83.80%),HE staining of tumor tissue(129 times,45.42%),and Animal epigenetic metrics(94 times,33.10%),and immunohistochemistry of tumor tissue(89 times,31.34%).ConclusionsAt present,osteosarcoma BALB/c-nu/nu mice aged 4 to 6 weeks are used as experimental animals,and human MG-63 cell heterotopic transplantation(back and axillary transplantation)is used to establish the animal model,and the detection indexes of osteosarcoma are comprehensively evaluated by animal apparent index,tumor apparent index and tumor histopathology.It is suggested to select serum biochemical index,apparent index of tumor tissue as well,HE staining of tumor tissue and immunohistochemistry of tumor tissue to evaluate the model.However,there is still a lack of animal model preparation and evaluation criteria with high clinical consistency.In this paper,the advantages and disadvantages are summarized through literaturemining and data analysis,in order to provide reference for the establishment of a good OS model and better application to OS mechanism research and new drug development.