SREBP2及PI3K/Akt/mTORC2在喉癌中的表达及相关性研究

Expression and correlation of SREBP2,PI3K/Akt/mTORC2 in laryngeal carcinoma

目的探究磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白C2(mTORC2)信号通路分子及人胆固醇调节元件结合蛋白2(SREBP2)分子在喉癌组织中的表达情况。方法选取本院2019年1月至2022年6月收治的原发性喉癌患者60例,取其肿瘤组织及癌旁组织样本,以免疫组织化学法测定PI3K、AKT、mTORC2及SREBP2表达情况并进行比较。结果肿瘤组织PI3K(88.3%vs.40.0%)、AKT(76.7%vs.31.7%)、mTORC2(78.3%vs.21.7%)、SERBP2(65.0%vs.8.3%)阳性表达率高于癌旁组织,差异均有统计学意义(P<0.05)。肿瘤直径大于或等于3 cm患者AKT、mTORC2、SERBP2阳性表达率高于肿瘤直径小于3 cm患者,差异均有统计学意义(P<0.05);临床分期Ⅲ~Ⅳ期患者AKT、SERBP2阳性表达率高于Ⅰ~Ⅱ期患者,差异均有统计学意义(P<0.05);不同分化程度患者mTORC2、SERBP2阳性表达率比较,差异均有统计学意义(P<0.05)。PI3K、AKT、mTORC2、SERBP2阳性表达患者2年生存率低于阴性表达患者,但差异无统计学意义(P>0.05)。结论喉癌患者肿瘤组织中PI3K/AKT/mTORC2信号通路分子及SREBP2均呈高表达,与肿瘤直径、临床分期及分化程度有相关性,是潜在的喉癌靶向代谢重编程治疗的靶点。

Objective To investigate the expression of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin C2(mTORC2)signal signaling and human cholesterol modulator junction albumin 2(SREBP2)in laryngeal carcinoma tissue.Methods A total of 60 primary laryngeal cancer patients with tumor and adjacent noncancerous tissue samples were collected and compared for PI3K,AKT,mTORC2,and SREBP2 expression by immunohistochemistry.Results The positive expressions of PI3K(88.3%vs.40.0%),AKT(76.7%vs.31.7%),mTORC2(78.3%vs.21.7%),and SREBP2(65.0%vs.8.3%)were higher in cancer tissues than those in adjacent tissues,the differences were statistically significant(P<0.05).The positive expressions of AKT,mTORC2 and SREBP2 were higher in patients whose tumors were≥3 cm in diameter than in those whose tumors were<3 cm in diameter,the differences were statistically significant(P<0.05).The positive expression rates of AKT and SREBP2 in patients with stagesⅢtoⅣdisease were higher than those with stagesⅠtoⅡdisease(P<0.05).The positive expression rate of mTORC2 and SREBP2 was different between patients with different degrees of differentiation(P<0.05).The 2-year survival rate of patients with positive expression of PI3K,AKT,mTORC2,and SREBP2 was lower than that with negative expression,although the differences were not statistically significant(P>0.05).Conclusion PI3K/AKT/mTORC2 are highly expressed in the cancerous tissues of laryngeal cancer,which are related to the tumor diameter,clinical stage and differentiation degree,and they are potential targets for metabolic reprogramming therapy of laryngeal cancer.