动脉Ⅲ号方对大鼠缺血下肢血管新生的影响

Effect of arterial No.Ⅲon angiogenesis of lower limbs in rat ischemia

目的:观察动脉Ⅲ号方对大鼠缺血下肢血管新生的影响。方法:选取雄性SD大鼠72只,在麻醉下结扎大鼠左下肢股动脉及其分支,并切断股动脉,制作下肢缺血模型。将制作成功的模型随机均分为模型组、动脉Ⅲ号方高、中、低剂量组和重组人粒细胞集落刺激因子(rhG-CSF)组,同时设假手术组(麻醉后暴露股动脉),每组12只。测量并比较各组大鼠的双下肢温差,大鼠外周血CD34阳性细胞比例,大鼠左下肢术后创面愈合情况及腓肠肌毛细血管数目。结果:与模型组比较,动脉Ⅲ号方给药组和rhG-CSF组能降低大鼠下肢温差(P<0.05),提高CD34阳性细胞比例(P<0.05),增加腓肠肌微血管数(P<0.05),促进创面愈合。结论:动脉Ⅲ号方能提高大鼠外周血CD34阳性细胞比例,促进缺血组织血管新生。

Objective To observe the effect of arterial No.III on ischemic lower limbs in rats.Methods Seventy-two male SD rats were selected to ligate the left lower limb femoral artery and its branches under anesthesia,and the femoral artery was cut off to make a model of lower limb ischemia.The successful models were randomly divided into model group,arterial No.III high,medium and low dose groups,granulocyte colony-stimulating factor(rhG-CSF)group,and sham surgery group(exposing the femoral artery after anesthesia),with 12 animals in each group.The temperature difference of both lower limbs of rats in each group was measured and compared,the proportion of CD34-positive cells in peripheral blood was detected and compared,and the wound healing of the left lower limb and the number of gastrocnemius muscle capillaries were observed and compared.Results Compared with the model group,the arterial No.III prescription dosing group and rhG-CSF group could reduce the temperature difference of the lower limbs(P<0.05),increase the proportion of CD34-positive cells,increase the number of microvessels of the gastrocnemius muscle(P<0.05),and promote wound healing.Conclusion Arterial No.III can increase the proportion of CD34-positive cells in peripheral blood of rats and promote angiogenesis in ischemic tissues.