欧李果浆对失眠小鼠的改善作用及机制

Ameliorative Effect and Mechanism of Cerasus humilis(Bge.)Sok on Insomnia in Mice

本研究对‘农大4号’‘农大7号’和‘燕山2号’欧李果浆进行体外模拟消化实验,选取γ-氨基丁酸(γ-aminobutyric acid,GABA)含量最高的‘农大4号’欧李果浆作为受试物,通过急性毒性试验、高架十字迷宫实验、戊巴比妥钠诱导下小鼠直接睡眠实验、睡眠时间延长实验以及巴比妥钠诱导下小鼠的睡眠潜伏期实验,探究欧李果浆对小鼠睡眠行为的改善作用。结果表明,灌胃800 mg/kg mb欧李果浆能够显著延长失眠小鼠的睡眠时长(P<0.05),提高小鼠入睡率,缩短睡眠潜伏期,且其改善作用可能与下调血清促炎因子水平和调节下丘脑-垂体-肾上腺轴功能有关。

The release characteristics ofγ-aminobutyric acid(GABA)from the fruits of‘Nongda 4’,‘Nongda 7’and‘Yanshan 2’Cerasus humilis(Bge.)Sok were studied during in vitro simulated digestion and‘Nongda 4’C.humilis(Bge.)Sok,with the highest GABA content,was selected to evaluate the improving effect of its fruit pulp on sleep behavior in mice by acute toxicity test,elevated cross maze test,pentobarbital sodium-indued immediate sleep test,prolonged sleep duration test,and sodium barbiturate-induced sleep latency test.The results showed that oral administration of 800 mg/kg mb C.humilis(Bge.)Sok.fruit pulp could significantly prolong the sleep duration of mice with insomnia(P<0.05),increase the sleep rate,and shorten the sleep latency.The ameliorative effect may be related to down-regulating the levels of proinflammatory factors and regulating the function of the hypothalamic-pituitary-adrenal axis.