核苷酸剪切修复基因mRNA和蛋白对头颈部鳞状细胞癌发生的预测价值研究

Predictive value of mRNA and protein of NER genes for occurrence of head and neck squamous cell carcinoma

目的:探讨核苷酸剪切修复(NER)基因mRNA和蛋白对头颈部鳞状细胞癌(HNSCC)发生的预测价值。方法:选取HNSCC患者98例为病例组,选取18岁及以上健康人群95例为对照组。采用荧光定量PCR实验和反相蛋白微阵列(RPPA)实验检测两组外周血淋巴细胞中9个核心NER基因mRNA和蛋白表达水平。采用Logistic回归分析HNSCC发生的影响因素。绘制受试者工作特征(ROC)曲线分析NER基因mRNA和蛋白表达对HNSCC发生的预测价值。结果:与对照组比较,病例组XPA mRNA和蛋白表达降低,XPB mRNA表达降低(均P<0.05)。XPA mRNA≤19.85和XPA蛋白<0.206是HNSCC发生的独立危险因素(均P<0.05)。单独XPA蛋白、XPA mRNA以及两者联合预测HNSCC发生的曲线下面积(AUC)分别为0.637、0.592、0.674,两者联合的AUC大于单项AUC(均P<0.05)。结论:XPA mRNA和蛋白表达水平是HNSCC发生的影响因素,XPA mRNA和蛋白联合预测HNSCC的发生较两者单独预测的价值更高。

Objective:To investigate the predictive value of mRNA and protein of nucleotide excision repair(NER)genes for occurrence of head and neck squamous cell carcinoma(HNSCC).Methods:A total of 98 HNSCC patients were selected as the case group,and 95 healthy people aged 18 years and over were selected as the control group.PCR and RPPA were used to detect the mRNA and protein expression levels of 9 core NER genes in peripheral blood lymphocytes of the two groups.Correlation of NER gene mRNA and protein expression level was analyzed by Spearman method.Logistic regression was used to analyze the influencing factors of HNSCC.ROC curve was drawn to analyze the predictive value of NER gene mRNA and protein expression for HNSCC.Results:Compared with the control group,the mRNA and protein expression of XPA and the mRNA expression of XPB were decreased in the case group(all P<0.05).XPA mRNA≤19.85 and XPA protein<0.206 were independent risk factors for HNSCC(both P<0.05).The AUC of XPA protein,XPA mRNA and their combination for predicting HNSCC were 0.637,0.592 and 0.674,respectively.The AUC of the combination of XPA protein and XPA mRNA was greater than that of each single item(all P<0.05).Conclusion:The expression levels of XPA mRNA and protein are risk factors for the occurrence of HNSCC.The combination of XPA mRNA and protein has a higher predictive value for the occurrence of HNSCC than either one alone.