外源丝氨酸对草菇退化菌株生产性状和营养成分的影响

Effect of exogenous serine on the productive traits and nutritional composition of degenerated strains of Volvariella volvacea

草菇不耐低温贮藏,因此其菌种退化现象频繁发生,阻碍了草菇产业的发展。丝氨酸(serine,Ser)作为一种功能性氨基酸,能有效调节机体的生理功能。该研究通过外源添加Ser,探讨其对草菇退化菌株生产性状和营养成分的影响。结果表明,外源Ser提高了草菇退化菌株的菌落直径和菌丝生长速度,缩短了原基形成时间和生产周期,提高了子实体直径和生物学效率,使退化严重的D3菌株恢复了出菇能力。而且,外源Ser有效提高了D2、D3菌株的主要营养成分含量。与对照组相比,Ser处理后的D2、D3菌株中粗多糖、粗蛋白、粗纤维、灰分、黄酮和多酚含量均显著增加;甲硫氨酸(methionine,Met)、半胱氨酸(cysteine,Cys)、Ser等氨基酸含量及Mg、Cu、Zn等矿物质含量也均显著提高。该研究可为草菇及其他食用菌退化菌种的复壮研究提供理论依据和技术支持。

Volvariella volvacea is not resistant to low temperature storage,which leads to frequent strain degeneration and hinders the development of V.volvacea industry.Serine(Ser) is a functional amino acid that effectively regulates the physiological functions of the body.In this study,the effect of exogenous addition of Ser on the productive traits and nutritional composition of degraded strains of V.volvacea was investigated.The results showed that exogenous Ser increased the colony diameter and mycelial growth rate of the degraded strain of V.volvacea,shortened the protoplast formation time and production cycle,improved the diameter and biological efficiency of the substrate,and restored the emergence capacity of the severely degraded D3 strain.Moreover,exogenous Ser effectively increased the content of the main nutrients in the D2 and D3 strains.Compared with the control group,the contents of crude polysaccharide,crude protein,crude fiber,ash,flavonoids and polyphenols in D2 and D3 strains treated with Ser increased significantly.The content of amino acids such as methionine(Met),cysteine(Cys),Ser and minerals such as Mg,Cu,and Zn were also significantly increased.This study can provide theoretical basis and technical support for the rejuvenation of degraded strains of V.volvacea and other edible fungi.