空心莲子草ApCAT基因家族鉴定和表达模式

Genome-wide identification and expression analysis of ApCAT gene family in Alternanthera philoxeroides

过氧化氢酶(CAT)是可清除生物体内多余的过氧化氢(H_(2)O_(2))并将其还原成水和氧气的氧化还原酶,在植物响应胁迫的过程中发挥多种功能,保护植物细胞免受活性氧毒害。目前,尚缺乏对空心莲子草(Alternanthera philoxeroides)CAT基因家族成员的系统研究。本研究通过生物信息学分析,从空心莲子草中鉴定到20个ApCAT基因,根据系统发育关系将其依次命名为ApCAT1至ApCAT20。特征分析表明,所有ApCATs均为亲水性蛋白。亚细胞定位预测发现,所有ApCATs均预测定位于过氧化物酶体,个别ApCATs也预测有定位线粒体、叶绿体和细胞核的可能。表达模式分析表明,ApCAT成员受纬度、海拔、温度、光照、水分条件等的影响较小,表达水平相对稳定。除草剂药效测试发现,20%氯氟吡氧乙酸和13%噁草酮的防治效果最好;结合定量PCR分析发现,ApCAT家族成员在空心莲子草响应除草剂处理过程中显著差异表达,参与对除草剂胁迫的应答过程。本研究系统分析了ApCATs家族成员的基本特征和表达模式,揭示了其除草剂诱导表达特征,为进一步研究ApCATs在空心莲子草响应除草剂胁迫中的生物学功能奠定了基础。

Catalase(CAT)is a REDOX enzyme that catalyzes the removal of excess hydrogen peroxide(H2O2)from living organisms and reduces H_(2)O_(2) to oxygen and water.CAT has a variety of functions in plant responses to various stresses,which helps protect plant cells from the toxicity of reactive oxygen species.Systematic research of CAT in Alternanthera philoxeroides has not been reported.In this study,20 ApCAT genes were identified by bioinformatics analysis.They were designated ApCAT1 to ApCAT20 according to their phylogenetic relationships.All 20 ApCATs were hydrophilic proteins.Subcellular localization predictions included the peroxisome for all ApCATs,with some ApCATs additionally predicted the in cell mitochondria,chloroplast,and nucleus.The analysis of expression patterns showed that ApCAT members were less affected by latitude,altitude,temperature,light and moisture conditions,and that their expression levels were relatively stable.Herbicide efficacy testing revealed the best control by 20% clofluropyloxyacetic acid and 13% oxalone.RT-qPCR analysis determined that ApCAT family members were significantly differentially expressed in response to herbicide treatment and participated in plant response to herbicide stress.The findings from the systematic analysis of the basic characteristics and expression patterns of ApCATs family members,and the herbicide induced expression profiles of ApCATs lay a foundation for further studies of the biological functions of ApCATs in the response to herbicide stress in A.philoxeroides.