鲍鱼壳对半水硫酸钙复合骨水泥的改性

Modification of calcium sulfate hemihydratecomposite bone cement by abalone shell

半水硫酸钙复合骨水泥因其良好的可塑性和原位自固化性,在临床上有广泛的应用,但是其降解产物呈酸性不利于后续的骨组织再生。鲍鱼壳的主要成分是碳酸钙,可有效地调控骨水泥降解后微环境的pH。又因为其含有有机质因子和微量元素,大大提高了骨水泥的生物相容性。通过在骨水泥中加入鲍鱼壳,可注射性可提高到97.20%±0.28%,这主要是由于鲍鱼壳粉末的加入,影响了骨水泥的水化过程,延长了凝固时间(可达26.93±0.31 min)。另一方面,鲍鱼壳粉末阻碍半水硫酸钙晶体长大,使得复合骨水泥中半水硫酸钙晶体变小(从1.42±0.77μm到0.90±0.36μm),有效地缓解了复合骨水泥降解过程中pH的下降(从6.08±0.06到6.93±0.02),极大地提高了细胞存活率(从87.00%±1.60%到96.41%±1.44%)。因此,研究表明鲍鱼壳的加入在临床上是一种潜在地提高骨水泥生物相容性的有效手段。

Calcium sulfate hemihydrate composite bone cement has been widely used in clinic because of its good plasticity and in situ self-curing properties.However,its degradation products are acidic and not conducive to subsequent bone tissue regeneration.The main component of abalone shell is calcium carbonate,which can effectively regulate the pH of the microenvironment after bone cement degradation.Because it contains organic factors and trace elements,it greatly improves the biocompatibility of bone cement.In this paper,by adding abalone shell to the bone cement,the injectable property could be increased to 97.20%±0.28%.This is mainly due to the addition of abalone shell powder,which affected the hydration process of the bone cement and prolongated the setting time(up to 26.93±0.31 min).On the other hand,abalone shell powder inhibited the growth of calcium sulfate hemihydrate crystals,which made the calcium sulfate hemihydrate crystals smaller(from 1.42±0.77μm to 0.90±0.36μm)in composite bone cement,and effectively alleviated the decrease of pH during the degradation of composite bone cement(from 6.08±0.06 to 6.93±0.02).The cell survival rate was greatly improved from 87.00%±1.60%to 96.41%±1.44%.Therefore,this study indicates that the addition of abalone shell is a potentially effective means to improve the biocompatibility of bone cement in clinical practice.