三亚地区2019-2020年急性肠胃炎患者A群双埃可病毒的分子特征

The molecular characteristics of Parechovirus A in patients with acute gastroenteritis in Sanya during 2019-2020

目的了解热带地区A群双埃可病毒(Parechovirus-A,PeV-A)流行率及其基因特征。方法2019年1月至2020年8月期间在三亚中心医院开展急性腹泻病监测,收集病例资料和患者粪便标本,采用巢式RT-PCR检测PeV-A,并对特定基因序列进行系统进化分析。结果纳入了2019年1月至2020年8月期间就诊的急性肠胃炎患者267例。PeV-A检出率为3.37%(9/267),检测到三种PeV-A基因型(HPeV1、HPeV3和HPeV5),它们与中国北京或中国香港的参考毒株关系最为密切。HPeV阳性患者主要为2岁以下幼儿,在夏季和冬季(分别为5月至8月和12月至2月),PeV-A的检出率较高。与PeV-A阴性腹泻患者相比,PeV-A阳性腹泻患者有更高比例的呼吸道感染、腹痛、呕吐和发烧。轮状病毒和诺如病毒是急性胃肠炎患者中最常见的与PeV-A共感染的病毒。结论PeV-A是三亚地区急性胃肠炎的重要病原体,流行的基因型与我国其他地区报道的相似。

Objective To invesigate the prevalence and genetic characteristics of Parechovirus A(PeV-A)in tropical areas.Methods Acute diarrhea disease surveillance was conducted at Sanya Central Hospital between January 2019 and August 2020.Patient data and fecal specimens were collected.PeV-A was detected by nested RT-PCR,and phylogenetic analyses of specific gene sequences were performed.Results Two hundred and sixty-seven patients with acute gastroenteritis were included in this study.The PeV-A detection rate was 3.37%(9/267),and three PeV-A genotypes(HPeV1,HPeV3,and HPeV5)were detectable,which were most closely related to the reference strains from either Beijing or Hong Kong.The HPeV-positive patients were predominantly young children under the age of 2 years.The detection rate of PeV-A was higher in summer and winter(May to August and December to February,respectively).Patients with PeV-A-positive diarrhea had a higher proportion of respiratory infections,abdominal pain,vomiting,and fever than those with PeV-A-negative diarrhea.Rotavirus and norovirus were the most common viruses co-infected with PeV-A in patients with acute gastroenteritis.Conclusions PeV-A is an important pathogen of acute gastroenteritis in Sanya,and its prevalent genotypes are similar to those reported in other regions of China.