宫颈癌患者血清WNT1诱导信号通路蛋白1、瞬时受体电位阳离子通道亚家族M成员7水平及临床意义

Levels and clinical significance of serum WNT1 inducible signaling pathway protein 1 and transient receptor potential cation channel subfamily M member 7 in patients with cervical cancer

目的探讨宫颈癌患者血清WNT1诱导信号通路蛋白1(WISP1)、瞬时受体电位阳离子通道亚家族M成员7(TRPM7)水平及临床意义。方法选取150例宫颈癌患者和164例宫颈上皮内瘤变(CIN)患者。采用酶联免疫吸附法检测两组患者血清WISP1水平,采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)法检测两组患者血清TRPM7 mRNA水平。宫颈癌发生的影响因素采用多因素Logistic回归分析。绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),评估血清TRPM7 mRNA、WISP1单独及联合检测对宫颈癌的诊断价值。结果宫颈癌患者血清TRPM7 mRNA、WISP1水平均明显高于CIN患者,差异均有统计学意义(P﹤0.01)。宫颈癌患者中文化程度为初中及以下、孕次≥3次、产次≥2次、合并高危人乳头瘤病毒(HPV)感染的比例均明显高于CIN患者,差异均有统计学意义(P﹤0.01)。多因素Logistic回归分析结果显示,TRPM7 mRNA水平升高、WISP1水平升高、合并高危HPV感染均是宫颈癌发生的独立危险因素(P﹤0.01)。ROC曲线显示,TRPM7 mRNA、WISP1联合检测诊断宫颈癌的AUC为0.944(95%CI:0.921~0.966),高于二者单独检测(P﹤0.05)。结论宫颈癌患者血清TRPM7 mRNA、WISP1水平较高,二者联合检测可提高对宫颈癌的诊断价值。

Objective To investigate the levels and clinical significance of serum WNT1 inducible signaling pathway protein 1(WISP1)and transient receptor potential cation channel subfamily M member 7(TRPM7)in patients with cervical cancer.Method A total of 150 patients with cervical cancer and 164 patients with cervical intraepithelial neoplasia(CIN)were selected.Serum WISP1 was detected by enzyme linked immunosorbent assay and the serum TRPM7 mRNA was detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Multivariate Logistic regression was used to analyze the factors influencing the occurrence of cervical cancer.The receiver operating characteristic(ROC)curve was drawn to calculate the area under the curve(AUC)to evaluate the diagnostic value of single and combined detection of TRPM7 mRNA and WISP1.Result The serum levels of TRPM7 mRNA and WISP1 in cervical cancer patients were significantly higher than those in CIN patients,the differences were statistically significant(P<0.01).The proportion of cervical cancer patients with education level of junior high school or below,pregnancy≥3 times,birth≥2 times,and concurrent high-risk human papilloma virus(HPV)infection were significantly higher than those of CIN patients,and the differences were statistically significant(P<0.01).Multivariate Logistic regression analysis showed that the increase of TRPM7 mRNA and WISP1 and high-risk HPV infection were all independent risk factors for cervical cancer(P<0.01).ROC curve showed that the AUC of combined TRPM7 mRNA and WISP1 was 0.944(95%CI:0.921-0.966),which was higher than the single detection(P<0.05).Conclusion The levels of serum TRPM7 mRNA and WISP1 in patients with cervical cancer are higher.The combined detection of WISP1 and TRPM7 mRNA can improve the diagnostic value of cervical cancer.