评价芝麻种质油分含量水平的SSR标记筛选与分析

Screening and Analysis of SSR Markers for Evaluating Sesame Germplasm Oil Content Level

为建立芝麻油分含量水平相关分子标记鉴定技术体系,利用自主开发的与芝麻油分含量紧密关联的24个SSR标记,对具有不同油分含量的48份芝麻种质进行了PCR快速鉴定和验证分析。结果表明,48份芝麻种质的油分含量在29.81%~57.86%,平均油分含量为46.91%。SSR标记的方差分析结果显示,24个SSR标记在48份样本中均表现出了多态性等位位点,其中Hs393(4/2)、Hs635(2/2)、Hs4082(2/1)和Hs4089(2/2)4个SSR标记多态性位点与芝麻油分含量显著(P<0.05)或极显著(P<0.001)关联。在检测的48个样本中,共有12个样本同时携带了上述4个SSR标记的优异等位位点,样本油分含量为52.74%~57.86%,均属于高油分含量样本。综上,Hs393、Hs635、Hs4082和Hs4089等4个SSR标记可以用于芝麻种质和育种材料的油分含量水平鉴定与评价,为建立并完善芝麻品质相关分子标记辅助育种技术体系提供了技术支撑。

In order to establish the molecular marker identification technology system related to sesame oil content level,we used 24 SSR markers closely related to sesame oil content developed by ourselves to conduct rapid PCR identification and validation analysis on 48 sesame germplasms with different oil content.The results showed that the oil content of 48 sesame germplasms ranged from 29.81%to 57.86%,with average oil content of 46.91%.The analysis of variance of SSR markers showed that 24 SSR markers showed polymorphic alleles in 48 samples,including Hs393(4/2),Hs635(2/2),Hs4082(2/1)and Hs4089(2/2).These four SSR marker polymorphic loci were significantly(P<0.05)or very significantly(P<0.001)associated with the sesame oil content level.Among the 48 samples tested,a total of 12 samples carried excellent alleles of the four SSR markers mentioned above,with oil content ranging from 52.74%to 57.86%,all of which belonged to high oil content samples.In conclusion,these four SSR markers,Hs393,Hs635,Hs4082,and Hs4089,can be used for identifying and evaluating the oil content level of sesame germplasms and breeding materials,providing technical support for establishing and improving the molecular marker⁃assisted breeding technology system related to sesame quality.