基于Plackett-Burman结合Box-Behnken优选经典名方三化汤挥发油β-环糊精包合工艺

Screen the Volatile Oilβ-Cyclodextrin Infused Process of the Classic Prescription Recipe Sanhua Decoction Based on Plackett-Burman Combined with Box-Behnken

目的优化经典名方三化汤挥发油饱和水溶液法包合工艺。方法以β-环糊精为包合材料,通过Plackett-Burman结合Box-Behnken设计试验,筛选三化汤总挥发油最佳包合工艺。采用显微镜法、薄层色谱法、紫外分光光度法对包合物进行验证,顶空-气相色谱-质谱联用确定包合前后挥发性成分变化。结果经典名方三化汤总挥发油最佳包合工艺为转速2000 r·min^(-1)、包合1 h、料液比1∶10、包合温度30℃、物料比1∶9。3次验证实验的综合评分分别为0.9810,0.9999,0.9634,平均值为0.9814,与模型预测值相差2.87%。结论饱和水溶液法包合工艺简单、稳定,挥发性成分包合前后基本不变,为三化汤开发及经典名方中挥发油包合工艺研究提供参考。

OBJECTIVE To optimize the cladding process of volatile oil saturated and aqueous solution method of classic prescription Sanhua decoction.METHODSβ-Cyclodextrin was used as the wrapping material,through Plackett-Burman combined with Box-Behnken design tests,the optimal packaging process of Sanhua decoction’s total volatile oil was screened.The clathrate was verified by microscopy,thin layer chromatography,and ultraviolet spectrophotometry,and the change of volatile composition before and after the envelope was determined by headspace-gas chromatography-mass spectrometry.RESULTS The best coating process of the total volatile oil of the classic prescription Sanhua decoction was as follows:speed 2000 r·min^(−1),wrapping 1 h,feed-liquid ratio 1∶10,baling temperature 30℃,material ratio 1∶9.The comprehensive scores of the three validation experiments were 0.9810,0.9999 and 0.9634,and the average value was 0.9814,which was 2.87%different from the predicted value of the model.CONCLUSION The saturated aqueous solution method is simple and stable,and the volatile components are basically unchanged before and after the encapsulation,which provides a reference for the development of Sanhua decoction and the research on the volatile oil in the classic recipe.