响应面法优化猫爪草多糖热浸提法提取工艺

Response Surface Methodology to Optimize the Extraction Process of Ranunculi ternati Radix Polysaccharides by Hot Extraction Method

[目的]优化猫爪草多糖热浸提法提取工艺。[方法]以猫爪草块根为原料,采用热水浸提法提取猫爪草多糖,选取提取时间、提取温度、液料比进行单因素试验,在单因素试验的基础上,以猫爪草的多糖得率作为响应值,运用响应面法对猫爪草多糖热浸提法提取工艺进行优化。[结果]热浸提法提取猫爪草多糖最佳提取工艺是提取时间2.16 h、提取温度82℃、液料比38∶1(mL∶g),在此条件下,猫爪草多糖得率最高,为3.160%。[结论]该研究为猫爪草多糖的药理作用研究和进一步开发应用提供理论依据。

[Objective]To optimize the extraction process of polysaccharides from Ranunculi ternati Radix by hot extraction method.[Method]Using the root tubers of Ranunculi ternati Radix as raw materials,the hot water extraction method was used to extract polysaccharides from Ranunculi ternati Radix.Single factor experiments were conducted by selecting extraction time,extraction temperature and liquid-solid ratio.Based on the single factor experiment,the polysaccharide yield of Ranunculi ternati Radix was used as the response value,and the response surface methodology was used to optimize the extraction process of Ranunculi ternati Radix polysaccharides using the hot water extraction method.[Result]The optimal extraction process for extracting polysaccharides from Ranunculi ternati Radix using hot extraction method was extraction time of 2.16 hours,extraction temperature of 82℃,and liquid-solid ratio of 38∶1(mL∶g).Under these conditions,the highest yield of Ranunculi ternati Radix polysaccharides was 3.160%.[Conclusion]This study provides a theoretical basis for the pharmacological study and further development and application of polysaccharides from Ranunculi ternati Radix.