离子液体1-庚基-3-甲基咪唑氯盐对小鼠的亚急性毒性

Sub-acute toxicity of ionic liquid 1-heptyl-3-methylimidazolium chloride in mice

目的:探究1-庚基-3-甲基咪唑氯盐(C7[MIM]Cl)对小鼠的亚急性毒性。方法:根据C7[MIM]Cl对小鼠的半数致死剂量(LD_(50))119.00 mg/kg,将24只SPF级昆明小鼠随机分为对照组,低(1/50 LD_(50),2.38 mg/kg)、中(1/20 LD_(50),5.95 mg/kg)和高(1/10 LD_(50),11.9 mg/kg)剂量C7[MIM]Cl染毒组,每组6只,每天灌胃1次,连续灌胃28 d,对照组灌胃给予等体积的生理盐水。记录小鼠摄食量和体质量变化情况,实验结束取小鼠心、肝、脾、肺、肾和卵巢,称取脏器质量并计算脏器系数,检测血清生化指标,并采用HE染色观察小鼠各脏器的病理变化。结果:与对照组比较,5.95 mg/kg C7[MIM]Cl染毒组小鼠饲料消耗量降低(P=0.05);血清生化指标表明2.38 mg/kg C7[MIM]Cl染毒组小鼠血清中谷丙转氨酶活性显著上升(P=0.049),而5.95 mg/kg组小鼠血清总胆红素、血尿素、尿酸和总胆固醇以及11.9 mg/kg组小鼠血糖水平显著下降(P<0.05);组织切片观察结果显示不同剂量C7[MIM]Cl亚急性染毒对小鼠的肝、脾、肺、肾以及卵巢均有一定程度的损伤,其中肝脏和肾脏损伤更为明显。结论:C7[MIM]Cl亚急性染毒可引起小鼠肝脏和肾脏的功能性损伤。

OBJECTIVE:To investigate sub-acute toxicity of the ionic liquid,1-heptyl-3-methy-limidazolium chloride,(C7[MIM]Cl)in mice.METHODS:According to the median lethal dose(LD_(50))of C7[MIM]Cl to mice as 119.00 mg/kg,Kunming mice(n=24)were randomly divided into blank control group and low,middle and high dose(1/50 LD_(50),1/20 LD_(50)and 1/10 LD_(50))groups(n=6 per group).Mice were treated with C7[MIM]Cl once a day by gavage for 28 days.The control group was given an equal volume of normal saline by gavage.The changes of food intake and body mass of mice were recorded;organ coefficients were calculated;serum biochemical indexes were detected;and the pathological changes of each organ were observed by H&E staining.RESULTS:The food intake in middle exposure group was lower than the control group(P=0.05).Serum biochemical indices showed that the activities of alanine aminotransferase(ALT)in the liver of the low exposure group was significantly increased(P=0.049).The total bilirubin(TBIL),UREA,uric acid(UA)and cholesterol(CHO)in the serum of the middle exposure group was significantly lower than the control(P<0.05).The glucose(GLU)level in the high dose group was significantly decreased(P<0.05).The observation of tissue section showed that different exposure doses induced a certain degree of damage to liver,spleen,lung,kidney and ovary.Especially,damages to the liver and kidney were more obvious.CONCLUSION:With sub-acute exposure of C7[MIM]Cl,liver and kidney were important target organs for damage from C7[MIM]Cl.