基于转录组学探讨海桐皮方提取膏外用治疗膝骨关节炎的作用机制

Study on the mechanism of haitongpi prescription extract ointment in the treatment of knee osteoarthritis based on transcriptome

目的:基于转录组学探讨海桐皮方提取膏外用治疗膝骨关节炎的作用机制。方法:将12只SPF级大鼠分为正常组(control,C组)、模型组(model,M组)和海桐皮方提取膏组(Haitongpi prescription,HP组),对M组和HP组通过Panicker法建立膝骨关节炎大鼠模型,并通过海桐皮方提取膏局部外用对HP组干预4周。提取小鼠膝关节软骨总RNA,并通过测序获得3组的差异基因。通过GO功能及KEGG通路富集分析对差异基因进行功能预测和分析。结果:C组vs M组共鉴定出差异基因109个,M组vs HP组鉴定出差异基因118个,共有基因28个。GO功能富集分析显示海桐皮方提取膏治疗膝骨关节炎的机制主要涉及免疫球蛋白介导的免疫应答、免疫球蛋白复合物和抗原结合等;KEGG通路富集分析显示与肿瘤坏死因子(tumor necrosis factor,TNF)信号通路、白细胞介素-17 (interleukin 17,IL-17)信号通路和雌激素信号通路相关。结论:海桐皮方提取膏可通过免疫球蛋白介导的免疫应答、免疫球蛋白复合物和抗原结合等机制,TNF信号通路、IL-17信号通路和雌激素信号通路等信号通路发挥对膝骨关节炎的治疗作用。

Objective To explore the mechanism of Haitongpi Prescription extract in the treatment of knee osteoarthritis based on transcriptome.Methods Total of 12 SPF grade rats were divided into control group(group C),model group(group M),and Haitongpi prescription group(group HP).The knee osteoarthritis rat model was established using the Panicker method for group M and group HP,and group HP was intervened by local topical application of Haitongpi Prescription extract for 4 weeks.Total RNA from mouse knee cartilage was extracted and three sets of differential genes were obtained through sequencing.Differential genes were prediction and analysis through GO function and KEGG pathway enrichment analysis.Results A total of 109 differentially expressed genes were identified in Group C versus Group M,while 118 differentially expressed genes were identified in Group M versus Group HP,resulting in a total of 28 genes.GO functional enrichment analysis showed that the mechanism of HP extract in treating knee osteoarthritis mainly involved immunoglobulin mediated immune response,immunoglobulin complexes,and antigen binding;KEGG pathway enrichment analysis showed correlation with tumor necrosis factor(TNF)signaling pathway,interleukin 17(IL-17)signaling pathway,and estrogen signaling pathway.Conclusion HP extract can exert therapeutic effects on knee osteoarthritis through mechanisms such as immunoglobulin mediated immune response,immunoglobulin complexes,and antigen binding,as well as signaling pathways such as TNF signaling pathway,IL-17 signaling pathway,and estrogen signaling pathway.