东方百合两个SERKs基因的克隆及表达分析

Cloning and Expression Analysis of Two SERKs Genes in Oriental Lily

体细胞胚发生类受体激酶(somatic embryo genesis receptor-like kinases,SERKs)能够感受并传递细胞外信号,在植物的生长发育等生理过程中起着重要作用。基于东方百合“西伯利亚”的转录组数据,克隆了两个百合SERK同源基因,LoSERK1和LoSERK2。LoSERK1的开放阅读框全长为1875 bp,编码624个氨基酸,包含53个潜在磷酸化位点。LoSERK2的开放阅读框全长为1887 bp,编码628个氨基酸,包含55个潜在磷酸化位点。LoSERKs与拟南芥、水稻、茶树等植物SERKs氨基酸序列相似性均超过90%。系统进化树分析结果表明,百合的LoSERKs与拟南芥的AtSERK1、AtSERK2及水稻的两个OsSERKs分在同一组。实时定量PCR分析表明,LoSERK1在花瓣及花药中的相对表达量都较高,而LoSERK2在开放的花瓣中相对表达含量较高,推测LoSERKs参与了花粉发育、花器官脱落功能的调控。

The ability of sensing and transmitting extracellular signals exhibited by somatic embryo genesis receptor-like kinases(SERKs)plays an important role in plant physiological processes such as growth and development.Base on the transcriptome data of Lilium oriental hybrids‘Siberia’,two SERK genes of Lilium,LoSERK1 and LoSERK2,have thus been coloned.The open reading frame of LoSERK1 has a total length of 1875 bp,encoding 624 amino acids and containing 53 potential phosphorylation sites,while the open reading frame of LoSERK2 has a total length of 1887 bp,encoding 628 amino acids and containing 55 potential phosphorylation sites.The amino acid sequence similarity exceeds 90%between LoSERKs and plant SERKs such as Arabidopsis thaliana,Oryza sativa and Camellia sinensis.The phylogenetic tree analysis shows that the LoSERKs of Lilium are in the same group as the AtSERK1 and AtSERK2 of Arabidopsis thaliana,and two OsSERKs of Camellia sinensis.A real time quantitative PCR analysis shows that LoSERK1 is characterzied with a high relative expression level in both petals and anthers,while the relative expression level of LoSERK2 is high in open petals.It is speculated that LoSERKs are involved in the regulation of pollen development andfloral organ abscission.