黄芩苷对感染性早产模型大鼠的干预效果及对AMPK/NF-κB通路的影响

Interventional effect of baicalin on infectious preterm model rats and impact on AMPK/NF-kB pathways

目的探究黄芩苷对感染性早产模型大鼠的干预效果及对腺苷酸活化蛋白激酶(AMPK)/核因子κB(NF-κB)通路的影响.方法选取成功妊娠Wistar雌性大鼠,8只为空白组,24只成功建模,分为模型组、药物对照组、实验组各8只.药物对照组、实验组分别进行黄体酮、黄芩苷干预,7 d后光镜下观察胎盘组织病理形态,酶联免疫吸附法检测炎性因子、基质金属蛋白酶(MMP)、氧化应激指标水平,实时荧光定量法和蛋白质免疫印迹法检测AMPK mRNA、NF-κB mRNA和蛋白表达量.结果与空白组相比,模型组、药物对照组、实验组单核细胞趋化蛋白1(MCP-1)、高迁移率族蛋白B1(HMGB1)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、基质金属蛋白酶-2(MMP-2)、MMP-9、丙二醛(MDA)水平、NF-κB mRNA和蛋白表达量上升,超氧化物歧化酶(SOD)水平、AMPK mRNA和蛋白表达量下降(P<0.05);与模型组相比,药物对照组、实验组MCP-1、HMGB1、IL-6、TNF-α、MMP-2、MMP-9、MDA水平、NF-κB mRNA和蛋白表达量逐渐下降,SOD水平、AMPK mRNA和蛋白表达量逐渐上升(P<0.05).结论黄芩苷可减轻感染性早产大鼠炎性及氧化应激反应,调节基质金属蛋白酶水平,其原因可能与AMPK/NF-κB通路得到调控有关.

OBJECTIVE To explore the interventional effect of baicalin on infectious preterm model rats and observe the impact on adenosine monophosphate-activated protein kinase(AMPK)/nuclear factor-kB(NF-kB)pathways.METHODS The pregnant Wistar rats were chosen as the study subjects,8 of which were assigned as the blank group,24 rats were successfully modeled and were divided into the model group,the drug control group and the experimental group,with 8 rats in each group.The drug control group and the experimental group were respectively intervened with progesterone and baicalin.The pathological features of placental tissues were observed under light microscopy after the intervention for 7 days,the levels of inflammatory factors,matrix metalloproteinase(MMP)and oxidative stress indexes were detected by enzyme-linked immunosorbent assay;the expression levels of AMPK mRNA,NF-kB mRNA and proteins were detected by real-time fluorescent quantitative method and Western blot.RESULTS The levels of monocyte chemotactic protein 1(MCP-1),high mobility group protein B1(HMGB1),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9)and malondialdehyde(MDA)was well as the expression levels of NF-kB mR-NA and proteins were higher in the model group,the drug control group and the experimental group than in the blank group;the superoxide dismutase(SOD)level and expression levels of AMPK mRNA and proteins were lower in the model group,the drug control group and the experimental group than in the blank group(P<o.o5).The levels of MCP-1,HMGB1,IL-6,TNF-α,MMP-2,MMP-9 and MDA as well as the expression levels of NFkB mRNA and proteins were lower in the drug control group and the experimental group than in the model group,while the SOD level and expression levels of AMPK mRNA were higher in the drug control group and the experimental group than in the model group(P<0.05).CONCLUSION Baicalin can alleviate the inflammatory response and oxidative stress response and regulate the matrix metalloproteinase level,which may be associated with the regulation of AMPK/NF-B pathways.