基于响应面法优化水鬼蕉中水仙环素的提取工艺

Optimization of Extraction Technology of Narciclasine from Hymenocallis Littoralis Based on Response Surface Method

目的通过响应面中心设计,获得水鬼蕉中水仙环素的最优提取工艺。方法选用昆明产水鬼蕉,在单因素实验基础上,选取温度、时间以及液料比3个因素结合Box-Behnken试验建立数学模型,分析考察3个因素对水仙环素提取率的影响程度,确定最适提取条件。结果二次多项式回归模型模型的F值为10.99,P值为0.0023,小于0.05,表明该模型具有统计学意义。失拟项P值为0.7568,表明模型显著且适当。最佳提取工艺为提取温度90.97℃,取时间4.13 h,液料比5.09 mL/g。水仙环素在此条件下的提取预测值、验证值分别为3.42×10^(-4)%、3.38×10^(-4)%。结论基于响应面中心设计优选的提取工艺稳定、可行、预测性良好,可用于水鬼蕉中活性成分的提取,为水鬼蕉资源开发和利用奠定基础。

Objective To obtain the optimal extraction process of narciclasine from Hymenocallis littoralis by response surface center design.Methods Based on the single factor experiment,temperature,time and liquidto-solid ratio were selected to establish a mathematical model combined with the Box-Behnken test.The influence degree of the three factors on the extraction rate of narciclasine was analyzed and investigated to determine the optimal extraction conditions.Results The optimum extraction conditions were as follows:extraction temperature 90.97℃,extraction time 4.13 h,liquid-solid ratio 5.09 mL∶1 g.The predicted and verified values of narciclasine extraction were 3.42×10^(-4)%and 3.38×10^(-4)%,respectively.Conclusion The optimized extraction process based on the response surface method is stable,feasible,and predictable,which could be used for the extraction of active components from Hymenocallis littoralis,and has laid the groundwork for the development and utilization of Hymenocallis littoralis.