浮床水芹不同器官酚酸类和脂肪酸类化感物质代谢组学分析

Metabolomics analysis of allelochemical phenolic acids and fatty acids in various organs of floating bed water dropwort

为明确浮床水芹不同器官化感物质特征与差异,揭示水芹化感抑藻机制,以浮床水芹为研究对象,基于液相色谱电喷雾质谱(LC-ESI-MS)代谢组学技术对不同发育阶段水芹种植水和整株不同器官的化感物质种类和含量进行分析。结果显示:在繁殖期、幼苗期和成熟期水芹种植水中检出酚酸类化感物质36种、脂肪酸类化感物质24种,且两类化感物质在幼苗期种植水中的相对含量显著高于繁殖期和成熟期。对幼苗期水芹根、茎、叶中酚酸类和脂肪酸类化感物质进行靶向分析,发现在各器官中共存的酚酸类化感物质有18种,其中阿魏酸和咖啡酸在叶片中含量较高,分别为(15.20±1.98)ng·mg^(-1)和(37.26±2.21)ng·mg^(-1);各器官中共存的脂肪酸类化感物质有33种,其中硬脂酸和肉豆蔻酸在根和叶中的含量较高,分别为(450.70±14.32)ng·mg^(-1)和(39.54±0.50)ng·mg^(-1)。研究表明,水芹种植水和其体内所含的酚酸类、脂肪酸类化感物质种类丰富,这些物质的释放是浮床水芹在富营养化水体中化感抑藻的主要原因。同时,与繁殖期和成熟期相比,幼苗期水芹叶片的酚酸类和脂肪酸类物质相对含量较高,是制备生物抑藻剂的良好材料。

To identify the characters and differences of the metabolites in different organs of water dropwort(Oenanthe javanica)and clarify the mechanism of allelopathy and algae inhibition of it,types and content of metabolites from different organs and culture water of floating bed water dropwort in different growth stages were analyzed using LC-ESI-MS metabolomics technology.In the culture water at the reproductive,seedling,and mature stages,36 different phenolic acids and 24 different fatty acids were identified.The relative contents of the two types of allelochemicals at the seedling stage were significantly higher than those at the reproductive and mature stages.Eighteen phenolic acids could be detected in all different organs via targeted metabolomics technology.Ferulic acid and caffeic acid contents in leaves were(15.20±1.98)ng·mg^(-1) and(37.26±2.21)ng·mg^(-1),respectively.Thirty-three different types of fatty acid allelochemicals could be detected in all organs.The contents of stearic acid and myristic acid in the roots and leaves were(450.70±14.32)ng·mg^(-1) and(39.54±0.50)ng·mg^(-1),respectively,much higher than that in stems.Taken together,a variety of phenolic acids and fatty acids were accumulated in both water dropwort plants and the culture water.The release of these compounds could inhibit the growth of algae in eutrophic water.The leaves of water dropwort at seedling stage could be ideal materials for biological algae inhibitors production due to the remarkable content of phenolic acids and fatty acids.