铁皮石斛的黄酮类成分测定及其生物可及性研究

Determination of flavonoids in Dendrobium officinale Kimura et Migo and study on its bioavailability

为探究铁皮石斛中黄酮类化合物的含量及其生物可及性,建立固相萃取超高效液相色谱串联质谱法测定17个黄酮单体的含量,并采用体外模拟消化法分析铁皮石斛在消化过程中黄酮类化合物的生物可及性。结果表明,铁皮石斛中总黄酮含量为1.52~4.36 mg·g^(-1),柚皮素、芦丁和夏佛塔苷是其中含量较高的3种黄酮单体。模拟胃小肠消化后,总黄酮的生物可及性为13.75%~21.45%,柚皮素、芦丁和夏佛塔苷的生物可及性分别为8.23%~18.37%、1.85%~22.87%和24.26%~42.65%;进一步结肠消化后,总黄酮的生物可及性为6.38%~14.13%,柚皮素、芦丁和夏佛塔苷的生物可及性分别为2.12%~5.67%、1.42%~5.70%和5.34%~13.33%,黄酮类化合物在胃小肠中的生物可及性大于结肠。铁皮石斛75%乙腈提取液的抗氧化能力>胃小肠消化液的抗氧化能力>结肠消化液的抗氧化能力,且抗氧化能力与黄酮类化合物的含量呈显著正相关。

A solid phase extraction-ultra performance liquid chromatography tandem mass spectrometry method was established to determine 17 flavonoid components in D.officinale.And meanwhile the in vitro method was used to simulate the digestive process and to analyze the bioavailability of flavonoids in D.officinale.The results showed that the total content of flavonoids in D.officinale was 1.52-4.36 mg·g^(-1).Naringenin,rutin and schaftoside were the three main flavonoid compounds in D.officinale.After gastrointestinal digestion,the bioavailability of total flavonoids was 13.75%-21.45%,and the bioavailability of naringin,rutin and schaftoside were 8.23%-18.37%,1.85%-22.87%and 24.26%-42.65%,respectively.After further colonic digestion,the bioavailability of total flavonoids was 6.38%-14.13%,and the bioavailability of naringin,rutin and schaftoside were 2.12%-5.67%,1.42%-5.70%and 5.34%-13.33%,respectively.The bioavailability of flavonoids in the gastrointestinal tract was greater than that in the colon phase.The antioxidant capacity followed D.officinale extract>gastrointestinal digestive juice>colon digestive juice,and the antioxidant capacity was positively correlated with the content of flavonoids.