基于全长转录组测序的稻秆潜蝇解毒代谢相关基因筛选

Screening for detoxification metabolism-related genes based on the full-length transcriptome sequencing of rice leaf miner Chlorops oryzae

为筛选水稻害虫稻秆潜蝇Chlorops oryzae潜在的解毒代谢酶基因,利用PacBio SequelⅡ测序平台对稻秆潜蝇幼虫进行全长转录组测序,基于测序结果筛选稻秆潜蝇的解毒代谢相关基因谷胱甘肽S-转移酶(glutathione S-transferase,GST)基因、羧酸酯酶(carboxylesterase,CarE)基因和细胞色素P450(cytochrome P450,CYP450)基因,并检测其在稻秆潜蝇不同发育阶段的相对表达量。结果表明,对稻秆潜蝇进行测序得到18100条去冗余转录本序列,共有16283条序列得到注释。通过比对分析共筛选出8条GST、12条CarE和28条CYP450基因序列,这些基因在稻秆潜蝇不同发育阶段的表达量具有显著差异,表明不同虫态的稻秆潜蝇其解毒代谢能力可能不同。此外,还筛选到1452个lncRNA序列,以及176个潜在的lncRNA靶基因序列,其中4个与解毒代谢基因相关。表明筛选获得的稻秆潜蝇解毒代谢酶基因及lncRNA靶基因可用于后续该虫的潜在抗药性研究及防治药剂筛选。

To screening for potential detoxification metabolism enzymes for the rice leaf miner Chlorops oryzae,the full-length transcriptome of C.oryzae larvae was obtained using the PacBio Sequel II sequencing platform.Based on the sequencing results,the genes related to detoxification metabolism such as glutathione S-transferases(GST),carboxylesterases(CarE)and cytochrome P450(CYP450)genes were screened.Moreover,the relative expressions of these genes at different developmental stages of C.oryzae were investigated.The results showed that a total of 18100 non-redundant transcripts were obtained,16283 of which were annotated.A total of eight GSTs,12 CarEs and 28 CYP450s were identified.The expressions of these genes were significantly different among developmental stages,implying that the detoxification capability of C.oryzae might be different in different insect developmental stages.In addition,1452 lncRNAs and 176 potential lncRNA target genes were identified;four target genes were associated with detoxification metabolic genes.All of these detoxification metabolism genes and lncRNA target genes can potentially be used for subsequent research on the resistance of this pest insect and screening for control agents.