饮水锶对高血压小鼠抗氧化功能的影响

EFFECT OF STRONTIUM IN DRINKING WATER ON ANTIOXIDANT FUNCTION IN HYPERTENSIVE MICE

目的探讨不同浓度锶对高血压小鼠抗氧化功能的影响,为防治人类高血压提供新思路。方法100只小鼠随机分为正常对照组(n=20)和造模组。正常对照组给予纯水,造模组给予2 mg/L N’-硝基-L-精氨酸甲酯盐酸盐(L-NAME),4w建立高血压小鼠模型。造模成功的80只小鼠随机分为模型对照组(n=20)、模型2.5mg/L锶水组(n=20)、模型5.0 mg/L锶水组(n=20)和模型10.0 mg/L锶水组(n=20),每2日监测小鼠饮水量和饲料消耗量,每周记录体重变化。10 w后测定小鼠主要脏器系数,血清和心脏中超氧化物歧化酶(T-SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、β-烟酰胺单核苷酸(NMN)和烟酰胺腺嘌呤二核苷酸(NAD+)含量。结果10 w后,5.0、10.0 mg/L锶水组小鼠血清和心脏SOD和GSH-Px活性分别为[(86.41±11.06)U/ml vs.(50.91±4.56)μmol/L vs.(7556.37±340.64)U/g tissue vs.(237.52±9.51)U/g tissue]和[(99.47±10.06)U/ml vs.(51.29±8.22)μmol/L vs.(7576.23±263.07)U/g tissue vs.(228.06±17.70)U/g tissue],较模型对照组明显上升[(69.47±12.45)U/ml vs.(43.95±8.02)μmol/L vs.(7165.82±715.19)U/g tissue vs.(228.06±17.70)U/g tissue,P<0.05]。10.0 mg/L锶水组小鼠血清CAT活性(7.17±0.55)U/ml较模型对照组(6.45±0.58)U/ml和5.0 mg/L锶水组(6.47±0.35)U/ml显著提高(P<0.05)。5.0、10.0 mg/L锶水组小鼠血清和心脏MDA含量分别为[(11.13±2.69)nmol/ml vs.(25.43±4.06)nmol/g]和[(11.63±2.14)nmol/ml vs.(27.74±4.37)nmol/g],较2.5 mg/L锶水组[(14.01±2.90)nmol/ml vs.(33.13±7.32)nmol/g]明显降低(P<0.05)。2.5 mg/L锶水组小鼠心脏NMN含量[(876.99±139.21)μg/g tissue]显著高于其他四组[(700.77±91.38)μg/g tissue vs.(797.70±76.44)μg/g tissue vs.(722.13±58.49)μg/g tissue vs.(761.05±55.97)μg/g tissue,P<0.05]。2.5、10.0 mg/L锶水组小鼠心脏NAD+含量分别为[(65.32±11.48)nmol/g和(73.68±10.39)nmol/g],较正常对照组(51.85±7.17)nmol/g和模型对照组(48.61±10.55)nmol/g显著提高(P<0.05)。结论饮水锶可以在一定程度上提高小鼠体内的抗氧化酶活性,降低氧化损伤程度,增加细胞的能量代谢,从而改善机体的氧化应激状态,提示锶可能有抗衰老、延长寿命的作用。

Objective To explore the effects of strontium in drinking water on blood pressure and antioxidant function in hypertensive mice,so as to provide new ideas for the prevention and treatment of hypertension.Methods A total of 100 mice were randomly divided into normal control group(n=20)and modeling group(n=80).Normal control group was given pure water,and modeling group was given 2 mg/L N'-nitro-L-arginine methyl ester hydrochloride(L-NAME)for 4 weeks to establish hypertensive model.The modeling mice were randomly divided into model control group(n=20),2.5 mg/L strontium water group(n=20),5.0 mg/L strontium water group(n=20)and 10.0 mg/L strontium water group(n=20).The water and food intake were recorded every two days,and body weight was recorded every week.After 10 w,the main organ coefficients,the contents of superoxide dismutase(T-SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),catalase(CAT),β-niacinamide mononucleotide(NMN)and niacinamide niacinamide adenine dinucleotide(NAD+)in the serum and serum and heart were determined.Results After 10w,SOD and GSH-Px activities in the serum and heart in 5.0 and 10.0 mg/L strontium water groups were((86.41±11.06)U/ml vsK(50.91±4.56)μmol/L,(7556.37±340.64)U/g tissue vsK(237.52±9.51)U/g tissue)and((99.47±10.06)U/ml vsK(51.29±8.22)μmol/L,(7576.23±263.07)U/g tissue vsK(228.06±17.70)U/g tissue),and significantly increased compared to the model control group((69.47±12.45)U/ml vsK(43.95±8.02)μmol/L,(7165.82±715.19)U/g tissue vsK(228.06±17.70)U/g tissue,P<0.05).CAT activity in the serum of 10.0 mg/L strontium water group(7.17±0.55)U/ml was significantly higher than that of the model control group(6.45±0.58)U/ml and 5.0 mg/L strontium water group(6.47±0.35)U/ml(P<0.05).MDA content in the serum and heart of 5.0 and 10.0 mg/L strontium water group were((11.13±2.69)nmol/ml vs.(25.43±4.06)nmol/g)and((11.63±2.14)nmol/ml vs.(27.74±4.37)nmol/g),and significantly decreased compared to 2.5 mg/L strontium water group((14.01±2.90)nmol/ml vs.(33.13±7.32)nmol/g,P<0.05).The content of NMN in the heart was significantly higher in 2.5 mg/L strontium water group(876.99±139.21)μg/g tissue than that in the other four groups((700.77±91.38)μg/g tissue,(797.70±76.44)μg/g tissue,(722.13±58.49)μg/g tissue and(761.05±55.97)μg/g tissue,P<0.05).The contents of NAD+in the heart of 2.5 and 10.0 mg/L strontium water groups were(65.32±11.48)nmol/g and(73.68±10.39)nmol/g,respectively and significantly increased compared to normal control group(51.85±7.17)nmol/g and model control group(48.61±10.55)nmol/g(P<0.05).Conclusion Strontium in drinking water can increase the activity of antioxidant enzymes in mice to a certain extent,reduce the degree of oxidative damage,and improve energy metabolism,suggesting that strontium may have anti-aging action.