大黄鱼虹彩病毒胞内增殖以及2个功能蛋白的细胞定位

Intracellular proliferation and localization of 2 functional proteins of large yellow croaker iridovirus

大黄鱼虹彩病毒病给大黄鱼养殖业可持续发展带来严重影响。本研究对分离自大黄鱼的虹彩病毒FD201807毒株在鳜鱼仔鱼细胞系1(MFF-1)上的增殖情况进行荧光定量分析。结果表明,MFF-1是适合FD201807毒株传代培养的细胞系。利用ISKNV-VP23鼠单抗和ISKNV-VP101兔多抗两种ISKNV蛋白的特异性抗体对感染了FD201807株的MFF-1进行细胞间接免疫荧光试验,结果显示FD201807株与ISKNV具有共同抗原,ISKNV-VP23的同源蛋白可能是膜结合蛋白,ISKNV-VP101的同源蛋白可能在胞质中组装。

Iridovirus disease has a significant impact on the sustainable development of large yellow croaker aquaculture.This study conducted fluorescence quantitative analysis on the proliferation of FD201807 strain of iridovirus isolated from large yellow croaker in the mandarin fish fry cell line 1(MFF-1).The results showed that MFF-1 is a suitable cell line for the passage culture of the FD201807 strain.Indirect immunofluorescence experiments were performed on FD201807-infected MFF-1 cells using specific antibodies against two ISKNV proteins,ISKNV-VP23 and ISKNV-VP101.The results demonstrated that FD201807 strain shares common antigens with ISKNV.The homologous protein of ISKNV-VP23 may be a membrane-binding protein,while the homologous protein of ISKNV-VP101 possibly assembles in the cytoplasm.