FABP4基因在3个贵州地方鸡种中多态性研究

Polymorphism and Bioinformatics Analysis of FABP4 Gene in Local Chickens of Guizhou

为系统筛查贵州地方鸡FABP4基因多态性。该试验以3个贵州地方鸡种(高脚鸡、百宜黑鸡和乌蒙凤鸡)为研究对象,构建品种DNA池,PCR扩增FABP4基因所有外显子及部分内显子区域,采用直接测序结合生物信息技术检测FABP4基因多态性。结果显示,在3个贵州地方鸡资源群体中共发现C51T和T1751C 2个SNPs位点,其中C51T位于第一外显子区,属于同义突变位点;等位基因频率估算发现,C51T位点在3个地方鸡种中多态丰度较高,T1751C位点在群体中多态丰度低;生物信息学得出,C51T位点突变后,鸡FABP4基因mRNA二级结构发生变化,自由能提升,稳定性降低。该试验成功挖掘出贵州地方鸡FABP4基因外显子及部分内含子区域变异位点,以期为贵州地方鸡分子标记开发利用提供参考。

In order to explore the polymorphisms of FABP4 gene among three breeds of chicken in Guizhou.This experiment build DNA pools for three species(Gaojiao chicken,Baiyi black chicken and Wumeng Crested chicken),amplify all exons and some introns ofFABP4gene by PCR and detected the polymorphism ofFABP4gene by direct sequencing combined with biological information technology.The results showed that two SNP loci(C51T and T1751C)were found in three breeds of chicken in Guizhou,one of which C51T was located in the first exon region and belonged to the synonymous mutation site.The allele frequency estimation found that the C51T has higher polymorphic abundance in three chicken breeds,and the T1751C was less polymorphic abundance.Using bioinformatics software analysis,it was found that after the C51T site mutation,the minimum free energy ofFABP4gene mRNA was improvedand and the stability was decreased.This study successfully screened the SNPs at all exons and some introns ofFABP4gene,and provide references for the development and utilization of molecular markers in local chicken in Guizhou.