裂褶菌产酶条件优化及对秸秆的降解

Optimization of enzyme production and straw degradation by Schizophyllum commune

对一株保存的裂褶菌Schizophyllum commune Fr.的产酶能力进行测定,在苯胺蓝和愈创木酚平板上出现褪色反应和红棕色氧化圈,表明该菌株具有较强产过氧化物酶和漆酶能力。在单因素试验和均匀试验的基础上,通过人工神经网络算法优化液体发酵的产酶条件,优化后漆酶酶活达到29.385 U/mL。应用玉米秸秆栽培裂褶菌,发现降解木质素的酶类主要为漆酶,在菌丝满袋时漆酶酶活达到最大48.333 U/mL,具有较强降解木质素能力,在工业、农业等方面具有较广阔的开发应用前景。

The enzyme production capacity of a strain of Schizophyllum commune was measured.Fading reaction and red brown oxidation ring of S.commune appeared on aniline blue and guaiacol plate indicated that the strain had strong ability to produce peroxidase and laccase.On the basis of single factor test and uniform test,artificial neural network algorithm was used to optimize the enzyme production conditions of liquid fermentation.The result showed that the optimized laccase activity reached 29.385 U/mL.The laccase activity reached the maximum of 48.333 U/mL in bagful time of cultivation using cornstalks as substrate.The lignin degrading enzymes were mainly laccase,which had broad development and application prospects in industry and agriculture.