山荆子类受体蛋白MbRLP7负调控杜梨对腐烂病的抗性

The Malus baccata Receptor Like Protein MbRLP7 Negatively Regulates the Resistance of Pyrus betulifolia Bunge to Valsa Canker

山荆子类受体蛋白MbRLP7为研究对象,结合生物信息分析、功能验证和表达量测定,分析了其在对腐烂病菌的抗性中的作用和调控机制。结果表明,MbRLP7编码的氨基酸序列与拟南芥AtRLP6和AtRLP7相似性最高,分别为43.0%和42.8%。MbRLP7启动子区域存在多个响应逆境相关信号的顺式作用元件,并响应腐烂病菌信号。将该基因导入杜梨悬浮细胞获得过表达细胞系3个。接种腐烂病病原菌Valsa pyri后,过表达细胞的发病速度显著高于野生型细胞。腐烂病菌代谢物处理后的所有过表达细胞系的细胞存活率显著低于野生型。此外,该基因的过表达增强了腐烂病菌代谢物对病原体相关分子模式(pathogen-associated molecular patterns,PAMPs)触发的免疫反应(PAMP-triggered immunity,PTI)、活性氧相关基因和病程相关蛋白PR1的诱导。综上所述,MbRLP7负调控杜梨对腐烂病的抗性,PTI、活性氧和PR1参与了该调控过程。

Based on bioinformatics analysis,functional verification,and expression measurement,the role of the receptor-like protein MbRLP7 in Valsa pyri resistance,and its potential regulatory mechanisms were explored.The results showed that MbRLP7 had the highest sequence similarity with AtRLP6 and AtRLP7,with 43.0%and 42.8%,respectively.There are several cis-elements in the promoter region of MbRLP7 in response to stress-related signals and Valsa pyri signals.The MbRLP7 was transformed into suspension cells of Pyrus betulifolia Bunge,and three overexpressed cell lines were obtained.After inoculation with the pathogen Valsa pyri(Vp),the growth rate of mycelia on the overexpressing cells was significantly higher than that on the wild-type cells.After Valsa pyri metabolites treatment,the cell activity of all overexpressed cell lines was significantly lower than that of wild-type.In addition,the overexpression of gene MbRLP7 enhanced the induction of Valsa pyri metabolites on PAMP-triggered immunity(PTI),reactive oxygen species related genes and pathogenesis-related protein PR1.In conclusion,MbRLP7 negatively regulates the resistance of Pyrus betulifolia Bunge to Valsa pyri,and PTI,reactive oxygen species and PR1 are involved in this regulatory process.