血浆胶质纤维酸性蛋白、α-突触核蛋白水平与帕金森病病情进展的相关性

Correlation between plasma glial fibrillary acidic protein,α-synuclein and progression of Parkinson disease

目的探讨帕金森病(Parkinson disease,PD)患者血浆胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、总α-突触核蛋白(α-synuclein,α-syn)、磷酸化-α-突触核蛋白(phosphorylated α-synuclein,p-α-syn)浓度与疾病进展的关系。方法连续收集就诊于淮安市第一人民医院神经内科门诊及住院的PD患者63例(PD组),同期选取年龄、性别相匹配的健康体检者25例为对照组(HC组),利用ELISA技术检测PD组和HC组血浆GFAP、α-syn、p-α-syn水平。使用SPSS 25.0软件进行统计分析。采用Mann-WhitneyU检验比较两组GFAP、α-syn、p-α-syn水平的差异,对PD组GFAP与α-syn、p-α-syn水平进行Spearman相关分析;对PD组不同Hoehn-Yahr分期的GFAP、α-syn、p-α-syn水平进行Mann-WhitneyU检验及Spearman相关分析。结果 PD组GFAP[0.80(0.62,0.97)ng/mL,0.54(0.27,0.88)ng/mL,Z=-3.216,P=0.001]、α-syn[3.93(3.16,6.02)ng/mL,2.67(1.74,4.47)ng/mL,Z=-2.600,P=0.009]、p-α-syn[5.80(1.31,15.62),0.71(0.61,0.83),Z=-6.607,P<0.001]水平高于HC组,差异有统计学意义。Spearman相关分析结果显示,PD组GFAP与α-syn(r=0.442,P<0.001)、p-α-syn(r=0.493,P<0.001)均呈正相关。PD中晚期组GFAP高于PD早期组,差异具有统计学意义(Z=-2.062,P=0.039);PD患者不同H-Y分期间α-syn与p-α-syn差异无统计学意义(P>0.05)。Spearman相关性分析显示,GFAP与H-Y分期呈正相关(r=0.277,P=0.018)。结论 PD患者血浆GFAP水平与疾病进展呈正相关,可作为PD病情评估的一个潜在的生物标志物。

Objective To investigate the correlation between plasma glial fibrillary acidic protein(GFAP),totalα-synuclein(α-syn),phosphorylatedα-synuclein(p-α-syn)and the disease progression in patients with Parkinson disease(PD).Methods Sixty-three patients with PD including the outpatients and inpatients from Department of Neurology of Huai'an First People's Hospital were continuously collected as a PD group,and 25 healthy people matched in age and sex were selected as a healthy control(HC)group at the same time.The plasma levels of GFAP,α-syn,and p-α-syn levels in the PD group and HC group were detected by ELISA.SPSS 25.0 software was used for statistical analysis.Mann-Whitney U test was performed to assess the differences of GFAP,α-syn and p-α-syn levels between the two groups,and the correlation between GFAP andα-syn and p-α-syn levels in PD group was examined by Spearman correlation analysis.The levels of GFAP,α-syn and p-α-syn in different Hoehn-Yahr(H-Y)stages of PD group were compared by Mann-Whitney U test and Spearman correlation analysis.Results The levels of GFAP(0.80(0.62,0.97)ng/mL,0.54(0.27,0.88)ng/mL,Z=-3.216,P=0.001),α-syn(3.93(3.16,6.02)ng/mL,2.67(1.74,4.47)ng/mL,Z=-2.600,P=0.009),and p-α-syn(5.80(1.31,15.62),0.71(0.61,0.83),Z=-6.607,P<0.001)in PD group were higher than those in HC group,and the difference was statistically significant.In PD group,there was a significant positive correlation between GFAP andα-syn(r=0.442,P<0.001)and p-α-syn(r=0.493,P<0.001).GFAP in the advanced PD group was higher than that in the early PD group,and the difference was statistically significant(P=0.039).There was no significant difference inα-syn and p-α-syn between different H-Y stages of PD patients(P>0.05).Plasma GFAP was positively correlated with H-Y stages(r=0.277,P=0.018).Conclusion The level of plasma GFAP in PD patients is positively correlated with disease progression,which can be used as a potential biomarker for PD disease assessment.