CircMATR3调节miR-129-5p/SOX2轴对鼻咽癌细胞CNE1恶性生物学行为的影响

Impact of CircMATR3 on the malignant biological behavior of nasopharyngeal carcinoma cells CNE1 by regulating miR-129-5p/SOX2 axis

目的:探讨CircMATR3调节miR-129-5p/SOX2轴对鼻咽癌细胞恶性生物学行为的影响。方法:以鼻咽癌细胞系CNE1为研究对象,将CNE1细胞分NC组、si-NC组、si-CircMATR3组、miR-NC组、miR-129-5p mimic组、si-CircMATR3+inhibitor NC组、si-CircMATR3+miR-129-5p inhibitor组、si-SOX2组。RT-qPCR检测细胞中CircMATR3、miR-129-5p、SOX2 mRNA的表达;Western blot检测蛋白表达;双荧光素酶报告实验验证CircMATR3与miR-129-5p、miR-129-5p与SOX2之间的靶向关系;克隆形成实验检测细胞克隆形成能力;Hoechst33342实验观察细胞形态学变化;Transwell小室实验检测细胞侵袭能力;流式细胞术检测细胞周期与细胞凋亡。结果:CircMATR3与miR-129-5p、miR-129-5p与SOX2之间存在靶向关系;沉默CircMATR3或过表达miR-129-5p或沉默SOX2可以抑制鼻咽癌细胞CNE1的克隆形成、侵袭和细胞周期转变,促进细胞凋亡和凋亡小体的形成;抑制miR-129-5p表达一定程度可以逆转沉默CircMATR3对于CNE1细胞的抑制作用。结论:沉默CircMATR3可以上调miR-129-5p表达,抑制SOX2表达,进而抑制鼻咽癌细胞CNE1的克隆形成、侵袭,促进细胞凋亡。

Objective:To investigate the impact of CircMATR3 on the malignant biological behavior of nasopharyngeal carcinoma cells by regulating miR-129-5p/SOX2 axis.Methods:Nasopharyngeal carcinoma CNE1 cells were separated into NC group,si-NC group,si-CircMATR3 group,miR-NC group,miR-129-5p mimic group,si-CircMATR3+inhibitor NC group,si-CircMATR3+miR-129-5p inhibitor group,and si-SOX2 group.RT-qPCR was applied to detect the expression of CircMATR3,miR-129-5p and SOX2 mRNA in cells.Western blot was applied to detect the protein expression.The targeting relationship between CircMATR3 and miR-129-5p,miR-129-5p and SOX2 was verified by double luciferase report experiment.Clonogenic assay was applied to detect the ability of cell cloning.Hoechst33342 assay was used to observe the morphological changes of cells.Transwell chamber test was applied to detect the cell invasion ability.Cell cycle and apoptosis was detected by flow cytometry.Results:There was a targeting relationship between CircMATR3 and miR-129-5p,between miR-129-5p and SOX2.Silencing CircMATR3 or overexpressing miR-129-5p or silencing SOX2 was able to inhibit the cloning and invasion and cell cycle transition of nasopharyngeal carcinoma cell CNE1,and promote cell apoptosis and apoptotic body formation.Inhibition of miR-129-5p expression could reverse the inhibition of silencing CircMATR3 on CNE1 cells to some extent.Conclusion:Silencing CircMATR3 can up regulate the expression of miR-129-5p and inhibit the expression of SOX2,thereby inhibiting the cloning and invasion of nasopharyngeal carcinoma cell CNE1 and promoting cell apoptosis.